Teshima H, Wanebo H, Pinsky C, Day N K
J Clin Invest. 1977 Jun;59(6):1134-42. doi: 10.1172/JCI108737.
The presence of circulating immune complexes in freshly drawn sera of patients with various forms of malignancies was detected by the 125I-Clq deviation test of Sobel et al. More than 50% of the 459 cancer sera showed a high inhibition of 125I-Clq uptake by sensitized sheep erythrocytes when compared with sera of 50 healthy laboratory personnel. The levels were compared with levels of total hemolytic complement and immunochemical determinations of Cl1 and C3. A correlation between high levels of circulating immune complexes and low levels of Clq was suggested. These immune complexes were separated by sucrose density gradient ultracentrifugation at low pH and were found to be heavier than 19S. Fluctuation of levels of immune complexes was evident when serial samples from the same patient were tested. Decrease of levels of immune complexes and a concomitant increase of Clq were detected after Calmette-Gueérin bacillus and autologous tumor cell treatment in some melanoma patients.
采用索贝尔等人的¹²⁵I-Clq偏离试验检测了各类恶性肿瘤患者新鲜血清中循环免疫复合物的存在情况。与50名健康实验室人员的血清相比，459份癌症患者血清中超过50%对致敏绵羊红细胞摄取¹²⁵I-Clq表现出高度抑制。将这些水平与总溶血补体水平以及Cl1和C3的免疫化学测定结果进行了比较。结果提示循环免疫复合物水平高与Clq水平低之间存在相关性。这些免疫复合物在低pH条件下通过蔗糖密度梯度超速离心进行分离，发现其分子量大于19S。对同一患者的系列样本进行检测时，免疫复合物水平的波动很明显。在一些黑色素瘤患者接受卡介苗和自体肿瘤细胞治疗后，检测到免疫复合物水平下降以及Clq随之升高。
