Gelidibacter maritimus sp. nov., isolated from marine sediment.

A Gram-stain-negative, yellow, strictly aerobic, non-flagellated, gliding, rod-shaped bacterial strain, was isolated from costal sediment, designated as F6074. The strain F6074 grows optimally at 30 °C, pH 7.5, and 3.0% (w/v) NaCl. Cells of strain F6074 are 0.2-0.5 µm wide and 1.0-2.0 µm long. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain F6074 belonged to the genus Gelidibacter, with the highest sequence similarity to Gelidibacter japonicus JCM 31967 (98.0%), followed by G. flavus JCM 31135 (97.7%), and similarity between strain F6074 and the type species G. algens DSM 12408 was 96.0%. Genome sequencing results revealed a genome size of 47,07,621 bp. The DNA G + C content was 37.8 mol%. The ANI and dDDH values between strain F6074 and G. japonicus JCM 31967 were 83.9 and 27.8%, the values between strain F6074 and G. algens DSM 12408 were 77.5% and 31.5%, and the values between strain F6074 and G. flavus JCM 31135 were 84.3 and 27.9%, respectively. The predominant quinone was MK-6 and the major fatty acids were iso-C, iso-CG, iso-C 3-OH, anteiso-C and summed feature 3. The polar lipids were consisted of phosphatidylethanolamine (PE), two unidentified aminolipids (AL) and three unidentified lipids (L1, L2, L3). Based on the phenotypic, phylogenetic and chemotaxonomic data, strain F6074 was considered to represent a novel species of the genus Gelidibacter, for which the name Gelidibacter maritimus sp. nov., is proposed. The type strain is F6074 (MCCC 1H00427 = KCTC 72942).