Marine College, Shandong University, Weihai, Shandong, 264209, People's Republic of China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, 266237, People's Republic of China.
Arch Microbiol. 2021 Oct;203(8):5117-5122. doi: 10.1007/s00203-021-02478-1. Epub 2021 Jul 26.
A Gram-stain-negative, yellow, strictly aerobic, non-flagellated, gliding, rod-shaped bacterial strain, was isolated from costal sediment, designated as F6074. The strain F6074 grows optimally at 30 °C, pH 7.5, and 3.0% (w/v) NaCl. Cells of strain F6074 are 0.2-0.5 µm wide and 1.0-2.0 µm long. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain F6074 belonged to the genus Gelidibacter, with the highest sequence similarity to Gelidibacter japonicus JCM 31967 (98.0%), followed by G. flavus JCM 31135 (97.7%), and similarity between strain F6074 and the type species G. algens DSM 12408 was 96.0%. Genome sequencing results revealed a genome size of 47,07,621 bp. The DNA G + C content was 37.8 mol%. The ANI and dDDH values between strain F6074 and G. japonicus JCM 31967 were 83.9 and 27.8%, the values between strain F6074 and G. algens DSM 12408 were 77.5% and 31.5%, and the values between strain F6074 and G. flavus JCM 31135 were 84.3 and 27.9%, respectively. The predominant quinone was MK-6 and the major fatty acids were iso-C, iso-CG, iso-C 3-OH, anteiso-C and summed feature 3. The polar lipids were consisted of phosphatidylethanolamine (PE), two unidentified aminolipids (AL) and three unidentified lipids (L1, L2, L3). Based on the phenotypic, phylogenetic and chemotaxonomic data, strain F6074 was considered to represent a novel species of the genus Gelidibacter, for which the name Gelidibacter maritimus sp. nov., is proposed. The type strain is F6074 (MCCC 1H00427 = KCTC 72942).
一株革兰氏阴性、黄色、严格需氧、无鞭毛、滑行、杆状的细菌菌株，从海岸沉积物中分离得到，命名为 F6074。该菌株 F6074 在 30°C、pH7.5 和 3.0%（w/v）NaCl 条件下生长最佳。F6074 菌株的细胞宽 0.2-0.5μm，长 1.0-2.0μm。基于 16S rRNA 基因序列的系统发育分析表明，菌株 F6074 属于 Gelidibacter 属，与 Gelidibacter japonicus JCM 31967 的序列相似度最高（98.0%），其次是 G. flavus JCM 31135（97.7%），与模式种 G. algens DSM 12408 的相似度为 96.0%。基因组测序结果显示，该菌株的基因组大小为 47,07,621bp，DNA G+C 含量为 37.8mol%。F6074 菌株与 G. japonicus JCM 31967 的 ANI 和 dDDH 值分别为 83.9%和 27.8%，与 G. algens DSM 12408 的 ANI 和 dDDH 值分别为 77.5%和 31.5%，与 G. flavus JCM 31135 的 ANI 和 dDDH 值分别为 84.3%和 27.9%。该菌株的主要醌类为 MK-6，主要脂肪酸为 iso-C、iso-CG、iso-C 3-OH、anteiso-C 和特征脂肪酸 3。极性脂包括磷脂酰乙醇胺（PE）、两种未鉴定的氨基脂（AL）和三种未鉴定的脂（L1、L2、L3）。基于表型、系统发育和化学分类学数据，菌株 F6074 被认为代表了 Gelidibacter 属的一个新种，建议将其命名为 Gelidibacter maritimus sp. nov.，模式菌株为 F6074（MCCC 1H00427=KCTC 72942）。
