College of Food and Biological Engineering, Jimei University, Xiamen, P. R. China.
Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, P. R. China.
J Sep Sci. 2020 Aug;43(16):3206-3215. doi: 10.1002/jssc.202000257. Epub 2020 Jul 8.
An effective two-dimensional liquid chromatography method has been established for the analysis of all-trans-astaxanthin and its geometric isomers from Phaffia rhodozyma employing a C18 column at the first dimension and a C30 column in the second dimension, connected by a 10-port valve using the photo-diode array detector. The regression equation of astaxanthin calibration curve was established, and the precision and accuracy values were found to be in the range of 0.32-1.14% and 98.21-106.13%, respectively. By using two-dimensional liquid chromatography, it was found that day light, ultrasonic treatment, and heat treatment have significant influence on the content of all-trans-astaxanthin in the extract from P. rhodozyma due to the transformation of all-trans-astaxanthin to cis-astaxanthin. The day light and ultrasonic treatments more likely transform all-trans-astaxanthin to 9-cis-astaxanthin, and the thermal treatment transforms all-trans-astaxanthin to 13-cis-astaxanthin. These results indicate that the two-dimensional liquid chromatography method can facilitate monitoring astaxanthin isomerization in the raw extract from P. rhodozyma. In addition, the study will provide a general reference for monitoring other medicals and bioactive chemicals with geometric isomers.
建立了一种有效的二维液相色谱法，用于分析红发夫酵母中全反式虾青素及其几何异构体。采用 C18 柱作为第一维，C30 柱作为第二维，通过 10 通阀连接，采用光电二极管阵列检测器。建立了虾青素校准曲线的回归方程，精密度和准确度值分别在 0.32-1.14%和 98.21-106.13%范围内。通过二维液相色谱法发现，由于全反式虾青素向顺式虾青素的转化，日光、超声处理和热处理对红发夫酵母提取物中全反式虾青素的含量有显著影响。日光和超声处理更可能将全反式虾青素转化为 9-顺式虾青素，而热处理将全反式虾青素转化为 13-顺式虾青素。这些结果表明，二维液相色谱法可以方便地监测红发夫酵母原始提取物中虾青素的异构化。此外，该研究将为监测其他具有几何异构体的医药品和生物活性化学品提供一般性参考。
