Corona-Cervantes Karina, García-González Igrid, Villalobos-Flores Loan Edel, Hernández-Quiroz Fernando, Piña-Escobedo Alberto, Hoyo-Vadillo Carlos, Rangel-Calvillo Martín Noé, García-Mena Jaime
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, CDMX, Mexico.
Departamento de Farmacología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, CDMX, Mexico.
PeerJ. 2020 May 22;8:e9205. doi: 10.7717/peerj.9205. eCollection 2020.
Human milk microbiota plays a role in the bacterial colonization of the neonatal gut, which has important consequences in the health and development of the newborn. However, there are few studies about the vertical transfer of bacteria from mother to infant in Latin American populations.
We performed a cross-sectional study characterizing the bacterial diversity of 67 human milk-neonatal stool pairs by high-throughput sequencing of V3-16S rDNA libraries, to assess the effect of the human milk microbiota on the bacterial composition of the neonate's gut at early days.
Human milk showed higher microbial diversity as compared to the neonatal stool. Members of the Staphylococcaceae and Sphingomonadaceae families were more prevalent in human milk, whereas the Pseudomonadaceae family, and genera were in the neonatal stool. The delivery mode showed association with the neonatal gut microbiota diversity, but not with the human milk microbiota diversity; for instance, neonates born by C-section showed greater richness and diversity in stool microbiota than those born vaginally. We found 25 bacterial taxa shared by both ecosystems and 67.7% of bacteria found in neonate stool were predicted to originate from human milk. This study contributes to the knowledge of human milk and neonatal stool microbiota in healthy Mexican population and supports the idea of vertical mother-neonate transmission through exclusive breastfeeding.
人乳微生物群在新生儿肠道细菌定植中发挥作用，这对新生儿的健康和发育具有重要影响。然而，关于拉丁美洲人群中细菌从母亲垂直传播到婴儿的研究较少。
我们进行了一项横断面研究，通过对V3-16S rDNA文库进行高通量测序，对67对人乳-新生儿粪便样本的细菌多样性进行表征，以评估早期人乳微生物群对新生儿肠道细菌组成的影响。
与人乳相比，新生儿粪便中的微生物多样性更高。葡萄球菌科和鞘脂单胞菌科的成员在人乳中更为普遍，而假单胞菌科及其属则在新生儿粪便中更为常见。分娩方式与新生儿肠道微生物群多样性有关，但与人乳微生物群多样性无关；例如，剖宫产出生的新生儿粪便微生物群的丰富度和多样性高于阴道分娩的新生儿。我们发现两个生态系统共有25个细菌分类群，预计新生儿粪便中67.7%的细菌来自人乳。这项研究有助于了解健康墨西哥人群中人乳和新生儿粪便微生物群，并支持通过纯母乳喂养实现母婴垂直传播的观点。
