Department of Anesthesiology, Xiangya Hospital Central South University, Xiangya Road 87#, Changsha, Hunan 410008, China.
Department of Urology, Second Xiangya Hospital, Central South University, Changsha, Hunan, China 410011., China.
Biomed Res Int. 2020 May 15;2020:6137947. doi: 10.1155/2020/6137947. eCollection 2020.
Nephrolithiasis is a frequent chronic urological condition with a high prevalence and recurrence rate. Proteomics studies on urolithiasis rat models are highly important in characterizing the pathophysiology of kidney stones and identifying potential approaches for preventing and treating kidney stones. The isobaric tags for relative and absolute quantification (iTRAQ) were performed to identify differentially expressed proteins (DEPs) in the kidney between urolithiasis rats and control rats. The results showed that 127 DEPs (85 upregulated and 42 downregulated) were identified in urolithiasis and control rats. The functions of DEPs were predicted by Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and protein-protein interaction (PPI) network analysis. The expression of four upregulated proteins (Tagln, Akr1c9, Spp1, and Fbn1) and four downregulated proteins (Hbb, Epb42, Hmgcs2, and Ca1) were validated by parallel reaction monitoring (PRM). Proteomics studies of ethylene glycol-induced urolithiasis rat models using iTRAQ and PRM helped to elucidate the molecular mechanism governing nephrolithiasis and to identify candidate proteins for the treatment of kidney stones.
肾结石是一种常见的慢性泌尿系统疾病，其患病率和复发率都很高。对尿石症大鼠模型进行蛋白质组学研究对于描述肾结石的病理生理学特征以及确定预防和治疗肾结石的潜在方法非常重要。采用同位素相对标记与绝对定量(iTRAQ)技术鉴定尿石症大鼠和对照组大鼠肾脏中的差异表达蛋白(DEPs)。结果表明，在尿石症和对照组大鼠中鉴定出 127 个 DEPs(85 个上调和 42 个下调)。通过基因本体论(GO)分析、京都基因与基因组百科全书(KEGG)富集分析和蛋白质-蛋白质相互作用(PPI)网络分析预测 DEPs 的功能。通过平行反应监测(PRM)验证了四个上调蛋白(Tagln、Akr1c9、Spp1 和 Fbn1)和四个下调蛋白(Hbb、Epb42、Hmgcs2 和 Ca1)的表达。使用 iTRAQ 和 PRM 对乙二醇诱导的尿石症大鼠模型进行蛋白质组学研究有助于阐明肾结石的分子机制，并鉴定出治疗肾结石的候选蛋白。
