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使用鸟枪法宏基因组学对越南宁顺省一个虾塘的微生物组数据集进行分析。

Microbiome dataset analysis from a shrimp pond in Ninh Thuan, Vietnam using shotgun metagenomics.

作者信息

Le Lan-Anh, Nguyen-Hoang Thien-Phuc, Huynh Van-Phuc, Nguyen Tan-Huy, Nguyen Thuy-Vy, Ho-Huynh Thuy-Duong

机构信息

Khoa Thuong Biotechnology Company Limited, No 17, Street 4, Phong Phu, Binh Chanh, Ho Chi Minh, Vietnam.

Department of Genetics, Faculty of Biology and Biotechnology, University of Science, VNU-HCM, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh, Vietnam.

出版信息

Data Brief. 2020 May 21;31:105731. doi: 10.1016/j.dib.2020.105731. eCollection 2020 Aug.

DOI:10.1016/j.dib.2020.105731
PMID:32509936
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7264489/
Abstract

Vietnam is one of the top shrimp producing and exporting countries in the world [1]. However, viral and bacterial epidemic diseases cause severe damages to shrimp farming, resulting in millions of US dollars losses annually [2]. Furthermore, inappropriate use of antibiotics in shrimp rearing lead to increased emergence of drug resistant pathogens [3]. Current practices for water quality control, mostly based on chemical and physical parameters; neglected biological criteria necessary for maintaining pond health. Ninh Thuan is a region situated in the South Central Coast of Vietnam. Due to its geographic location, a large part of this region is dedicated to shrimp ( post-larvae production and rearing. This article presents a microbiome dataset from two water samples collected in a shrimp rearing pond in Ninh Thuan. We used Oxford Nanopore Technologies (ONT) for metagenomic sequencing of the samples to characterize microbial communities and antibiotic resistance profiles. The metagenome dataset generated will provide an understanding and comparison framework of the microbial diversity and functionality among shrimp ponds with potential application in health management and shrimp rearing industry.

摘要

越南是世界上最大的虾类生产和出口国之一[1]。然而,病毒和细菌流行病对虾类养殖造成了严重破坏,每年造成数百万美元的损失[2]。此外,在虾类养殖中不当使用抗生素导致耐药病原体的出现增加[3]。目前的水质控制做法大多基于化学和物理参数,而忽视了维持池塘健康所需的生物学标准。宁顺是位于越南中南部海岸的一个地区。由于其地理位置,该地区的很大一部分用于虾类(幼体生产和养殖)。本文展示了从宁顺一个虾类养殖池塘采集的两个水样的微生物组数据集。我们使用牛津纳米孔技术(ONT)对样本进行宏基因组测序,以表征微生物群落和抗生素抗性谱。生成的宏基因组数据集将为虾塘之间的微生物多样性和功能提供一个理解和比较框架,在健康管理和虾类养殖行业具有潜在应用价值。

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本文引用的文献

1
Evaluating antimicrobial resistance in the global shrimp industry.评估全球虾类养殖业中的抗菌素耐药性。
Rev Aquac. 2020 May;12(2):966-986. doi: 10.1111/raq.12367. Epub 2019 Jul 8.
2
NanoPack: visualizing and processing long-read sequencing data.NanoPack:可视化和处理长读测序数据。
Bioinformatics. 2018 Aug 1;34(15):2666-2669. doi: 10.1093/bioinformatics/bty149.
3
Benchmarking of methods for identification of antimicrobial resistance genes in bacterial whole genome data.细菌全基因组数据中抗菌药物耐药基因鉴定方法的基准测试。
J Antimicrob Chemother. 2016 Sep;71(9):2484-8. doi: 10.1093/jac/dkw184. Epub 2016 Jun 30.
4
The metagenomics RAST server - a public resource for the automatic phylogenetic and functional analysis of metagenomes.宏基因组学RAST服务器——用于宏基因组自动系统发育和功能分析的公共资源。
BMC Bioinformatics. 2008 Sep 19;9:386. doi: 10.1186/1471-2105-9-386.