Viac J, Bustamante R, Thivolet J
Br J Dermatol. 1977 Jul;97(1):1-10. doi: 10.1111/j.1365-2133.1977.tb15421.x.
The identification of mononuclear cells extracted from cutaneous tumours (basal cell carcinoma, squamous cell carcinoma and superficial, spreading melanoma) has been investigated. The relative numbers of T cells and B cells have been determined using the E-rosette test and the EAC-rosette test. The results have been compared to those of delayed hypersensitivity type reactions. Different cell distribution patterns (E/EAC ratio) have been found in the infiltrates according to the type of tumour. An immunocytochemical technique has been developed for the identification in situ of immunoglobulin-producing cells in the inflammatory infiltrates. In each case the class of immunoglobulin (IgM, IgG or IgA) has been identified and the relative frequency of Ig-producing cells has been determined. The results indicate humoral and cellular immune responses with variations attributable to the type of tumour. In weakly malignant tumours, the infiltrate is characterized by an elevated number of T lymphocytes and numerous plasma cells which secrete all classes of Ig; in highly malignant tumours it is characterized by a reduced number of both T lymphocytes (E rosette) and plasma cells which do not secrete all classes of Ig.
对从皮肤肿瘤（基底细胞癌、鳞状细胞癌和浅表扩散性黑色素瘤）中提取的单核细胞进行了鉴定研究。使用E花环试验和EAC花环试验确定了T细胞和B细胞的相对数量。将结果与迟发型超敏反应类型的结果进行了比较。根据肿瘤类型，在浸润物中发现了不同的细胞分布模式（E/EAC比率）。已开发出一种免疫细胞化学技术，用于原位鉴定炎症浸润物中产生免疫球蛋白的细胞。在每种情况下，都确定了免疫球蛋白的类别（IgM、IgG或IgA），并确定了产生Ig细胞的相对频率。结果表明存在体液免疫和细胞免疫反应，且这些反应因肿瘤类型而异。在低度恶性肿瘤中，浸润物的特征是T淋巴细胞数量增加以及分泌所有类别的Ig的大量浆细胞；在高度恶性肿瘤中，其特征是T淋巴细胞（E花环）数量减少以及不分泌所有类别的Ig的浆细胞数量减少。
