Grossi-Oliveira Gustavo, Faverani Leonardo P, Mendes Bruno Coelho, Braga Polo Tárik Ocon, Batista Mendes Gabriel Cury, de Lima Valthierre Nunes, Ribeiro Júnior Paulo Domingos, Okamoto Roberta, Magro-Filho Osvaldo
Oral and Maxillofacial Surgery, Diagnostic and Surgery, Aracatuba School of Dentistry, Sao Paulo State University (UNESP), Aracatuba, Sao Paulo, Brazil.
Oral and Maxillofacial Surgery, Department of Oral Surgery, University of Sagrado Coracao, Bauru, Sao Paulo, Brazil.
Int J Biomater. 2020 May 23;2020:5182845. doi: 10.1155/2020/5182845. eCollection 2020.
This study evaluated the osteoconductive potential of four biomaterials used to fill bone defects. For this, 24 male Albino rabbits were submitted to the creation of a bilateral 8 mm calvarial bone defect. The animals were divided into four groups-bovine hydroxyapatite, Bio-Oss® (BIO); Lumina-Bone Porous® (LBP); Bonefill® (BFL); and an alloplastic material, Clonos® (CLN)-and were euthanized at 14 and 40 days. The samples were subjected to histological and histometric analysis for newly formed bone area. Immunohistochemical analysis for Runt-related transcription factor 2 (Runx2), vascular endothelial growth factor (VEGF), and osteocalcin (OC) was performed. After statistical analysis, the CLN group showed greater new bone formation (NB) in both periods analyzed ( < 0.05). At 14 days, the NB showed greater values in BIO in relation to LBP and BFL groups; however, after 40 days, the LBP group surpassed the results of BIO ( < 0.001). The immunostaining showed a decrease in Runx2 intensity in BIO after 40 days, while it increased for LBP ( < 0.05). The CLN showed increased OC compared to the other groups in both periods analyzed ( < 0.05). Therefore, CLN showed the best osteoconductive behavior in critical defects in rabbit calvaria, and BFL showed the lowest osteoconductive property.
本研究评估了用于填充骨缺损的四种生物材料的骨传导潜力。为此，将24只雄性白化兔双侧颅骨制造8毫米骨缺损。动物被分为四组——牛羟基磷灰石、Bio-Oss®（BIO）；Lumina-Bone Porous®（LBP）；Bonefill®（BFL）；以及一种异体材料Clonos®（CLN）——并在14天和40天时实施安乐死。对样本进行组织学和组织计量学分析以测定新形成骨面积。对 runt 相关转录因子2（Runx2）、血管内皮生长因子（VEGF）和骨钙素（OC）进行免疫组织化学分析。经过统计分析，CLN组在两个分析时间段均显示出更大的新骨形成（NB）（<0.05）。在14天时，与LBP和BFL组相比，BIO组的NB值更高；然而，在40天后，LBP组超过了BIO组的结果（<0.001）。免疫染色显示40天后BIO组Runx2强度降低，而LBP组则增加（<0.05）。在两个分析时间段，CLN组的OC均高于其他组（<0.05）。因此，CLN在兔颅骨临界缺损中表现出最佳的骨传导行为，而BFL表现出最低的骨传导性能。
