Villalobos Guiehdani, Muñoz-García Claudia I, Rodríguez-Cabo-Mercado Roberto, Mendoza-Bazán Nancy, Hernández-Ortiz Adrián, Villanueva-García Claudia, Martínez-Hernández Fernando, Rendón-Franco Emilio
Departamento de Ecología de Agentes Patógenos, Hospital General "Dr. Manuel Gea González", Calzada de Tlalpan # 4800, Del. Tlalpan, Mexico City C.P. 14080, Mexico.
Departamento de Producción Agrícola y Animal, Universidad Autónoma Metropolitana, Calzada del Hueso 1100, Col. Villa Quietud, Del. Coyoacán, Mexico City C.P. 04960, Mexico.
Pathogens. 2020 Jun 12;9(6):464. doi: 10.3390/pathogens9060464.
More than 180 mammalian species have been found naturally infected with . Many of them play an important role in the maintenance of this parasite. In particular, new studies have appeared which indicate that some species of Procyonidae family may play a role as hosts, however, more data are needed to evaluate their long-term physiological response to parasite infection, especially for specific antibodies. In this study, antibodies to were detected and prevalence and epitope recognition were assessed by ELISA (using discrete typing unit (DTU) I as antigen) and WB (using DTU I and DTU II as antigens) and sera from two procyonid species obtained through five-year follow-up of two semicaptive populations living in the same habitat. Marked heterogeneity in antigens recognition between species and differences in seroprevalence ( = 0.0002) between white-nosed coatis (), 51.8% (115/222), and common raccoons (), 28.3% (23/81), were found. Antigens with high molecular weight when DTU-I was used were the most recognized, while a greater antigen diversity recognition was observed with DTU-II; for white-nosed coatis, low-molecular-weight antigens were mainly recognized, while for common raccoons proteins with molecular weights greater than 80 kDa were recognized most. These divergent humoral immune responses could be related to an alleged pattern of recognition receptors and major histocompatibility complex molecules difference in the procyonids species.
已发现180多种哺乳动物自然感染了[寄生虫名称未给出]。其中许多在这种寄生虫的维持中起着重要作用。特别是，新的研究表明，浣熊科的一些物种可能作为[宿主类型未给出]宿主发挥作用，然而，需要更多数据来评估它们对寄生虫感染的长期生理反应，尤其是针对特定抗体的反应。在本研究中，通过ELISA（使用离散分型单元（DTU）I作为抗原）和WB（使用DTU I和DTU II作为抗原）检测了针对[寄生虫名称未给出]的抗体，并评估了流行率和表位识别，所用血清来自对生活在同一栖息地的两个半圈养种群进行五年随访获得的两种浣熊科物种。发现物种之间在抗原识别上存在明显的异质性，白鼻浣熊（[白鼻浣熊学名未给出]）的血清阳性率为51.8%（115/222），普通浣熊（[普通浣熊学名未给出]）的血清阳性率为28.3%（23/81），两者之间的血清阳性率存在差异（P = 0.0002）。使用DTU-I时，高分子量抗原最易被识别，而使用DTU-II时观察到更大的抗原多样性识别；对白鼻浣熊而言，主要识别低分子量抗原，而对普通浣熊来说，分子量大于80 kDa的蛋白质最易被识别。这些不同的体液免疫反应可能与浣熊科物种中所谓的识别受体模式和主要组织相容性复合体分子差异有关。
