Wei Pengtao, Yang Jing, Zhang Dandan, Cui Meng, Li Lianjun
Department of Urology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang 471000, People's Republic of China.
Central Sterile Supply Department, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China.
Onco Targets Ther. 2020 May 21;13:4523-4531. doi: 10.2147/OTT.S246274. eCollection 2020.
Increasing evidence has shown that abnormally expressed long non-coding RNA (lncRNA) plays crucial roles in prostate cancer (PCa) progression.
Here, we analyzed the expression level of lncRNA HAND2 antisense RNA 1 (HAND2-AS1) in PCa cells and tissues. Function assays were performed to investigate the biological roles of HAND2-AS1 in PCa cell behaviors. Bioinformatics methods, luciferase activity reporter assay, and RNA pull-down assay were performed to validate the connection of microRNA-106a-5p (miR-106a-5p) with HAND2-AS1. Also, the target of miR-106a-5p was explored using the same methods.
Our results revealed HAND2-AS1 expression was decreased in both PCa cells and tissues. In vitro functional assays showed HAND2-AS1 could inhibit PCa cell proliferation and colony formation through promoting cell apoptosis. Dual-luciferase activity assays showed miR-106a-5p could directly bind with HAND2-AS1 and RNA binding motif protein 24 (RBM24). Mechanistically, we showed that HAND2-AS1 regulates PCa cell behaviors via targeting miR-106a-5p/RBM24 axis.
In summary, our results illustrated that HAND2-AS1 functions as miR-106a-5p sponge to regulate RBM24 expression, and to influence PCa progression.
越来越多的证据表明，异常表达的长链非编码RNA（lncRNA）在前列腺癌（PCa）进展中起关键作用。
在此，我们分析了lncRNA HAND2反义RNA 1（HAND2-AS1）在PCa细胞和组织中的表达水平。进行功能试验以研究HAND2-AS1在PCa细胞行为中的生物学作用。采用生物信息学方法、荧光素酶活性报告基因试验和RNA下拉试验来验证微小RNA-106a-5p（miR-106a-5p）与HAND2-AS1的联系。此外，使用相同方法探索miR-106a-5p的靶标。
我们的结果显示HAND2-AS1在PCa细胞和组织中的表达均降低。体外功能试验表明HAND2-AS1可通过促进细胞凋亡来抑制PCa细胞增殖和集落形成。双荧光素酶活性试验表明miR-106a-5p可直接与HAND2-AS1和RNA结合基序蛋白24（RBM24）结合。从机制上讲，我们表明HAND2-AS1通过靶向miR-106a-5p/RBM24轴来调节PCa细胞行为。
总之，我们的结果表明HAND2-AS1作为miR-106a-5p的海绵体来调节RBM24表达，并影响PCa进展。
