长链非编码 RNA HAND2-AS1 通过减少 MSC 来源的外泌体 miR-106a-5p 的分泌抑制三阴性乳腺癌的生长。

LncRNA HAND2-AS1 suppressed the growth of triple negative breast cancer via reducing secretion of MSCs derived exosomal miR-106a-5p.

机构信息

Department of Obstetrics and Gynecology, Shanghai Eighth People Hospital, Xuhui, Shanghai, China.

Department of General Surgery, Shanghai Eighth People Hospital, Xuhui, Shanghai, China.

出版信息

Aging (Albany NY). 2020 Dec 3;13(1):424-436. doi: 10.18632/aging.202148.

BACKGROUND

Triple-negative breast cancer (TNBC) is a special type of breast cancer, its tumor cell metastasis rate is much higher than other types, and at the same time has a high rate of postoperative recurrence, which significantly threatens the health of women. Thus, it is urgent to explore a new treatment for TNBC.

RESULTS

MiR-106a-5p was up-regulated in TNBC tissues and cells, and was positively correlated with the tumor grade, which indicated poor prognosis in TNBC patients. Mesenchymal stem cells (MSCs) can transport miR-106a-5p into TNBC cells via exosomes. Functional analysis showed exo-miR-106a-5p secreted by MSCs promoted tumor progression in TNBC cells. Furthermore, lncRNA HAND2-AS1 inhibited miR-106a-5p levels, and HAND2-AS1 was decreased in TNBC tissues and cells. Besides, overexpression of HAND2-AS1 reduced the secretion of exo-miR-106a-5p secretion from MSCs, thus suppressed TNBC development.

CONCLUSION

Our study revealed that HAND2-AS1 inhibited the growth of TNBC, which were mediated by the inhibitory effects of MSC-derived exosomal miR-106a-5p.

背景

三阴性乳腺癌（TNBC）是一种特殊类型的乳腺癌，其肿瘤细胞转移率远高于其他类型，同时术后复发率较高，严重威胁着女性的健康。因此，迫切需要探索 TNBC 的新治疗方法。

结果

miR-106a-5p 在 TNBC 组织和细胞中上调，并与肿瘤分级呈正相关，这表明 TNBC 患者的预后不良。间充质干细胞（MSCs）可以通过外泌体将 miR-106a-5p 转运至 TNBC 细胞。功能分析表明，MSCs 分泌的 exo-miR-106a-5p 促进了 TNBC 细胞的肿瘤进展。此外，lncRNA HAND2-AS1 抑制 miR-106a-5p 的水平，HAND2-AS1 在 TNBC 组织和细胞中减少。此外，过表达 HAND2-AS1 减少了 MSC 来源的外泌体 miR-106a-5p 的分泌，从而抑制了 TNBC 的发展。