Alizadeh Negar, Salimi Abdollah, Sham Tsun-Kong, Bazylewski Paul, Fanchini Giovanni
Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran.
Department of Chemistry, University of Western Ontario, 1151 Richmond St., N6A 5B7 London, Ontario, Canada.
ACS Omega. 2020 May 19;5(21):11883-11894. doi: 10.1021/acsomega.9b03252. eCollection 2020 Jun 2.
Artificial enzyme mimics have gained considerable attention for use in sensing applications due to their high stability and outstanding catalytic activity. We show that cerium oxide nanosheets (NSs) exhibit triple-enzyme mimetic activity. The oxidase-, peroxidase-, and catalase-like activities of the proposed nanoparticles are demonstrated using both colorimetric and electron paramagnetic resonance (EPR) spectroscopy. On the basis of the excellent catalytic activity of cerium oxide NSs toward hydrogen peroxide, an electrochemical approach for the high-throughput detection of HO in living cells was established. This report presents an analytical microfluidic chip integrated with a cerium oxide NS mimic enzyme for the fabrication of a simple, sensitive, and low-cost electrochemical sensor. Three Au microelectrodes were fabricated on a glass substrate using photolithography, and the working electrode was functionalized using cerium oxide NSs. The operation of this biosensor is based on cerium oxide NSs and presents a high sensitivity over a wide detection range, between 100 nM and 20 mM, with a low detection limit of 20 nM and a high sensitivity threshold of 226.4 μA·cm·μM. This microfluidic sensor shows a strong response to HO, suggesting potential applications in monitoring HO directly secreted from living cells. This sensor chip provides a promising platform for applications in the field of diagnostics and sensing.
人工酶模拟物因其高稳定性和出色的催化活性而在传感应用中受到了广泛关注。我们表明氧化铈纳米片（NSs）具有三重酶模拟活性。通过比色法和电子顺磁共振（EPR）光谱法证明了所提出的纳米颗粒具有类似氧化酶、过氧化物酶和过氧化氢酶的活性。基于氧化铈NSs对过氧化氢的优异催化活性，建立了一种用于高通量检测活细胞中HO的电化学方法。本报告介绍了一种集成了氧化铈NS模拟酶的分析微流控芯片，用于制造一种简单、灵敏且低成本的电化学传感器。使用光刻技术在玻璃基板上制备了三个金微电极，并使用氧化铈NSs对工作电极进行了功能化处理。这种生物传感器的操作基于氧化铈NSs，在100 nM至20 mM的宽检测范围内具有高灵敏度，检测限低至20 nM，灵敏度阈值高达226.4 μA·cm·μM。这种微流控传感器对HO表现出强烈响应，表明其在直接监测活细胞分泌的HO方面具有潜在应用。这种传感器芯片为诊断和传感领域的应用提供了一个有前景的平台。
