Çolak Bayram, Aksoy Faruk, Yavuz Selman, Demircili Mehmet Emin
Department of General Surgery, Selcuk University School of Medicine, Konya, Turkey.
Department of General Surgery, Necmettin Erbakan University School of Medicine, Konya, Turkey.
Turk J Surg. 2019 Dec 16;35(4):314-320. doi: 10.5578/turkjsurg.4354. eCollection 2019 Dec.
Various scolicidal agents are applied for the destruction of protoscolices in cysts media. Undesirable complications of the scolicidal agents limit the techniques to treat the cyst disease. Therefore, new non-toxic scolicidal agents are needed. Upon laser light irradiation, the photothermal gold nanoparticles (AuNPs) convert the absorbed laser light into heat through photothermal effect which kills the surrounding protoscolices by rising the temperature of the cysts media. In this study, we introduced biocompatible AuNPs as a non-toxic scolicidal agent to cure liver hydatid cysts.
The protoscoleces were collected from the livers of naturally infected sheeps. In each experimental group, 1.5 mL suspensions of hydatid liquid containing protoscolices were added to test tubes. The test tubes were divided into five groups. Control, AuNPs only, Green laser only, High-dose AuNPs + laser and Low-dose AuNPs + laser groups. Two concentrations (0.4 and 0.8 mL) of AuNPs and three laser powers (30, 50, 150 mW) were applied for 30, 60 and 120 minutes to the groups. Then the ciysts liquid assessed under a light microscope and determined the viability of protoscoleces.
Protoscolices in high-dose AuNPs group were destructed up to 89.30% deaths under 150 mW laser power for 120 minutes. However, negligible cell deaths were observed in cases where only AuNPs added or only laser irradiated groups. Increasing the dose of AuNPs or laser power or duration of aplication increased the protoscolosidal death rate.
In the study, we have successfully demonstrated that the AuNPs are an effective therapeutic and scolicidal agent to cure hydatid cyst disease under laser irradiation.
多种杀头节剂被用于破坏囊肿介质中的原头节。杀头节剂的不良并发症限制了治疗囊肿疾病的技术。因此，需要新型无毒杀头节剂。在激光照射下，光热金纳米颗粒（AuNPs）通过光热效应将吸收的激光转化为热量，通过升高囊肿介质的温度杀死周围的原头节。在本研究中，我们引入生物相容性AuNPs作为一种无毒杀头节剂来治疗肝包虫囊肿。
从自然感染绵羊的肝脏中收集原头节。在每个实验组中，将1.5 mL含有原头节的包虫液悬浮液加入试管中。试管分为五组：对照组、仅AuNPs组、仅绿色激光组、高剂量AuNPs + 激光组和低剂量AuNPs + 激光组。将两种浓度（0.4和0.8 mL）的AuNPs和三种激光功率（30、50、150 mW）分别作用于各组30、60和120分钟。然后在光学显微镜下评估囊肿液并确定原头节的活力。
在150 mW激光功率下照射120分钟，高剂量AuNPs组的原头节破坏率高达89.30%。然而，在仅添加AuNPs或仅激光照射的组中观察到可忽略不计的细胞死亡。增加AuNPs剂量、激光功率或作用持续时间可提高原头节死亡率。
在本研究中，我们成功证明了AuNPs在激光照射下是治疗包虫囊肿疾病的有效治疗剂和杀头节剂。
