Institute of Quality Standardization & Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, 100081, Beijing, China.
Key Laboratory of Agrofood Safety and Quality (Beijing), Ministry of Agriculture, 100193, Beijing, China.
J Agric Food Chem. 2020 Jul 15;68(28):7298-7315. doi: 10.1021/acs.jafc.0c01962. Epub 2020 Jul 2.
Acetylcholinesterase inactivating compounds, such as organophosphate (OP) and carbamate (CM) pesticides, are widely used in agriculture to ensure sustainable production of food and feed. As a consequence of their applications, they would result in neurotoxicity, even death. In this essence, the development of enzyme inhibition methods still shows great significance as rapid detection techniques for on-site large-scale screening of OPs and CMs. Initially, mechanisms and applications of various enzyme-inhibition-based methods and devices, including optical colorimetric assay, fluorometric assays, electrochemical biosensors, rapid test card, and microfluidic device, are highlighted in the present overview. Further, to enhance the enzyme sensitivity for detection; alternative enzyme sources or high yield enrichment methods (such as abzyme, artificial enzyme, and recombinant enzyme), as well as enzyme reactivation and identification, are also addressed in this comprehensive overview.
乙酰胆碱酯酶失活化合物，如有机磷 (OP) 和氨基甲酸酯 (CM) 农药，广泛应用于农业以确保食物和饲料的可持续生产。由于它们的应用，会导致神经毒性，甚至死亡。因此，作为现场大规模筛选 OP 和 CM 的快速检测技术，酶抑制方法的开发仍然具有重要意义。本文首先重点介绍了各种基于酶抑制的方法和装置的原理和应用，包括光学比色测定法、荧光测定法、电化学生物传感器、快速检测卡和微流控装置。此外，为了提高检测的酶灵敏度；还讨论了替代酶源或高产富集方法（如抗体酶、人工酶和重组酶）以及酶的再激活和鉴定。
