Doctoral School of Health Sciences, Faculty of Health Sciences, University of Pécs, Pécs, Hungary.
Department of Obstetrics and Gynaecology, Medical School, University of Pécs, Pécs, Hungary.
Int Breastfeed J. 2020 Jun 17;15(1):57. doi: 10.1186/s13006-020-00301-z.
The profile of sirtuin 3 (SIRT3), 8-hydroxy-2'-deoxyguanosine (8-OHdG), brain-derived neurotrophic factor (BDNF) and serotonin (5-HT) in cord blood and in early breast milk was studied and it was related to perinatal factors. 5-HT and BDNF signalling systems have been claimed to play a critical role in intrauterine development, postnatal adaptation and lactation. Since prematurity and Caesarean birth are frequently associated with inflammation and related oxidative stress, an attempt was made to reveal the adaptive changes of the protective SIRT3 and the complex interplay among these bioactive components in cord blood and early breast milk.
Three groups each consisting of 30 mothers were included in the study: mothers who underwent spontaneous vaginal birth at term (group I), Caesarean section at term (group II) and preterm birth (group III). Venous cord blood and early breast milk samples were collected for measuring the biomarkers. SIRT3, 8-OHdG, BDNF and 5-HT levels were determined by using commercially available ELISA kits.
It was demonstrated that cord blood levels of SIRT3, BDNF and 5-HT were markedly reduced whereas those of 8-OHdG were significantly elevated after preterm birth when compared with birth at term. The Caesarean section was associated with a moderate decrease in BDNF and 5-HT, however, both SIRT3 and 8-OHdG remained unaffected. Breast milk levels of all biomarkers studied proved to be independent of their corresponding cord blood concentrations. In response to preterm birth breast milk SIRT3, 8-OHdG and 5-HT increased significantly, while a drastic fall occurred in BDNF. A significant positive relationship was found of 5-HT with SIRT3 and 8-OHdG irrespective of the gestational age and the mode of delivery.
It is suggested that the selected biomarkers in the breast milk mostly derive from local production by the mammary glands and 5-HT may have an essential role in the control of this process.
研究了脐带血和早期母乳中沉默调节蛋白 3（SIRT3）、8-羟基-2'-脱氧鸟苷（8-OHdG）、脑源性神经营养因子（BDNF）和 5-羟色胺（5-HT）的特征，并将其与围产期因素相关联。5-HT 和 BDNF 信号系统被认为在宫内发育、产后适应和哺乳中发挥关键作用。由于早产和剖宫产通常与炎症和相关氧化应激有关，因此试图揭示保护性 SIRT3 的适应性变化以及这些生物活性成分在脐带血和早期母乳中的复杂相互作用。
本研究纳入了每组各 30 名母亲的三个组：足月自然阴道分娩的母亲（I 组）、足月剖宫产的母亲（II 组）和早产的母亲（III 组）。采集静脉脐带血和早期母乳样本以测量生物标志物。使用市售的 ELISA 试剂盒测定 SIRT3、8-OHdG、BDNF 和 5-HT 水平。
与足月出生相比，早产儿的脐带血 SIRT3、BDNF 和 5-HT 水平显著降低，而 8-OHdG 水平显著升高。剖宫产与 BDNF 和 5-HT 中度降低相关，但 SIRT3 和 8-OHdG 不受影响。研究中所有生物标志物的母乳水平均与其相应的脐带血浓度无关。与早产相比，母乳中的 SIRT3、8-OHdG 和 5-HT 显著增加，而 BDNF 则急剧下降。无论胎龄和分娩方式如何，5-HT 均与 SIRT3 和 8-OHdG 呈显著正相关。
建议母乳中的选定生物标志物主要来自乳腺的局部产生，5-HT 可能在控制这一过程中发挥重要作用。
