D'Abadia PatrÍcia L, BailÃo Elisa FlÁvia Luiz C, Lino JÚnior Ruy S, Oliveira Matheus Gabriel, Silva Vinicius B, Oliveira Leandra A R, ConceiÇÃo Edemilson C, Melo-Reis Paulo Roberto, Borges Leonardo Luiz, GonÇalves Pablo JosÉ, Almeida Luciane M
Universidade Estadual de Goiás, Rodovia BR 153, nº 3105, Fazenda Barreiro do Meio, Campus Henrique Santillo, 75132-400 Anápolis, GO, Brazil.
Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Rua 235, s/n, Setor Leste Universitário, 74605-050 Goiânia, GO, Brazil.
An Acad Bras Cienc. 2020 Jun 12;92(2):e20190107. doi: 10.1590/0001-3765202020190107. eCollection 2020.
The Hancornia speciosa latex reveals angiogenic, osteogenic, and anti-inflammatory properties, which present its potential for developing of wound healing drugs; however, the latex compounds responsible for angiogenesis remain unknown. One strategy to screen these active compounds is evaluation of latex fractions. This study aimed to obtain different fractions of latex and evaluate its angiogenic activity separately using the chick chorioallantoic membrane (CAM) assay. The serum (SE) fraction was responsible for angiogenesis, which was subject to biochemical characterization and computational simulations in order to understand the contribution of H. speciosa latex in wound healing process. Our results revealed weak antioxidant potential and absence of antimicrobial activity in the SE fraction. Phytochemical analysis identified chlorogenic acids (CGA) as the main compound of SE fraction. CGA bioactivity predictions identify different molecules associated with extracellular matrix (ECM) remodeling, such as metalloproteinases, which also are overexpressed in our CAM assay experiment. Docking simulations revealed the interactions between CGA and matrix metalloproteinase 2. In conclusion, SE latex fraction stimulates angiogenesis and may influence ECM remodeling. These properties may contribute to the wound healing process, and also confirm the widespread use of this plant.
汉科尼亚胶乳具有促血管生成、成骨和抗炎特性，这表明其在开发伤口愈合药物方面具有潜力；然而，负责血管生成的胶乳化合物仍然未知。筛选这些活性化合物的一种策略是评估胶乳组分。本研究旨在获得不同的胶乳组分，并使用鸡胚绒毛尿囊膜（CAM）试验分别评估其血管生成活性。血清（SE）组分负责血管生成，对其进行了生化表征和计算模拟，以了解汉科尼亚胶乳在伤口愈合过程中的作用。我们的结果显示，SE组分的抗氧化潜力较弱且无抗菌活性。植物化学分析确定绿原酸（CGA）为SE组分的主要化合物。CGA生物活性预测识别出与细胞外基质（ECM）重塑相关的不同分子，如金属蛋白酶，在我们的CAM试验中这些分子也过表达。对接模拟揭示了CGA与基质金属蛋白酶2之间的相互作用。总之，SE胶乳组分刺激血管生成并可能影响ECM重塑。这些特性可能有助于伤口愈合过程，也证实了这种植物的广泛用途。
