Dairy Research Institute, IPLA-CSIC, Paseo Rio Linares s/n, 33300 Villaviciosa, Spain; Molecular Microbiology Group, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Dairy Research Institute, IPLA-CSIC, Paseo Rio Linares s/n, 33300 Villaviciosa, Spain.
Food Chem. 2020 Nov 30;331:127303. doi: 10.1016/j.foodchem.2020.127303. Epub 2020 Jun 11.
β-phenylethylamine and tryptamine are biogenic amines (BA) often found in foods. In general, BA are assumed to be toxic and their accumulation in food is not recommended. However, present knowledge regarding the toxicity of β-phenylethylamine and tryptamine is limited; more information is needed if qualitative and quantitative risk assessments of foods are to be successfully conducted. This study describes a real-time analysis of β-phenylethylamine and tryptamine toxicity on a human intestinal epithelial cell line. Both BA caused cell necrosis and apoptosis, although the former was the main mode of action of β-phenylethylamine, and the latter the main mode of action of tryptamine. Only tryptamine was cytotoxic at concentrations found in BA-rich foods. The results presented in this work may contribute to establish legal limits for β-phenylethylamine and tryptamine in food.
β-苯乙胺和色胺是生物胺（BA），常存在于食物中。一般来说，BA 被认为是有毒的，不建议在食物中积累。然而，目前关于 β-苯乙胺和色胺毒性的知识有限；如果要成功地对食物进行定性和定量风险评估，需要更多的信息。本研究描述了一种实时分析 β-苯乙胺和色胺对人肠上皮细胞系毒性的方法。两种 BA 都会导致细胞坏死和凋亡，尽管前者是 β-苯乙胺的主要作用模式，后者是色胺的主要作用模式。只有色胺在 BA 丰富的食物中发现的浓度下具有细胞毒性。本工作中提出的结果可能有助于为食物中的 β-苯乙胺和色胺建立法定限量。
