Del Rio Beatriz, Redruello Begoña, Linares Daniel M, Ladero Victor, Fernandez Maria, Martin Maria Cruz, Ruas-Madiedo Patricia, Alvarez Miguel A
Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300 Villaviciosa, Spain.
Food Chem. 2017 Mar 1;218:249-255. doi: 10.1016/j.foodchem.2016.09.046. Epub 2016 Sep 8.
Tyramine and histamine are the biogenic amines (BA) most commonly found at high concentrations in food; they may even appear together at toxic concentrations. The present work examines, via real-time cell analysis, whether histamine and tyramine show synergistic toxicity towards intestinal cell cultures. Employing a constant equipotency ratio, their interaction was examined via the combination index (CI) method of Chou & Talalay. Co-treatment with tyramine and histamine was associated with a stronger cytotoxic effect than was treatment with either BA or on its own. Indeed, a synergistic interaction (CI<1) was observed in the range of concentrations found in foods. The results also show that histamine, at concentrations below the legal limit, increases the cytotoxicity of tyramine at concentrations frequently reached in some foods. The synergistic cytotoxicity of tyramine and histamine should be taken into account when establishing legal limits designed to ensure consumer safety.
酪胺和组胺是在食物中最常发现的高浓度生物胺；它们甚至可能以有毒浓度同时出现。本研究通过实时细胞分析，探究组胺和酪胺对肠道细胞培养物是否具有协同毒性。采用恒定等效应比，通过Chou和Talalay的联合指数（CI）法研究它们的相互作用。酪胺和组胺联合处理比单独使用任何一种生物胺处理都具有更强的细胞毒性作用。实际上，在食物中发现的浓度范围内观察到了协同相互作用（CI<1）。结果还表明，在低于法定限量的浓度下，组胺会增加某些食物中经常达到的酪胺浓度的细胞毒性。在制定旨在确保消费者安全的法定限量时，应考虑酪胺和组胺的协同细胞毒性。
