National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China; Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs, Hangzhou, 310008, China.
National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China.
Plant Physiol Biochem. 2020 Sep;154:195-203. doi: 10.1016/j.plaphy.2020.06.005. Epub 2020 Jun 9.
Calcineurin B-like (CBL) proteins, a class of Ca-binding proteins, play vital roles in calcium signal transduction by interacting specifically with CBL-interacting protein kinases (CIPKs), and these two gene families and their interacting complexes are involved in regulating plant responses to various environmental stimuli. In the present study, eight CBL and 25 CIPK genes were identified in tea plant and divided into four and five subfamilies, respectively. Analysis of the expression of these genes in response to abiotic stresses (mature leaves treated with cold, salinity, and PEG and young shoots treated with cold) revealed that CsCBL1/3/5 and CsCIPK1/4/5/6a/7/8/10b/10c/12/14a/19/23a/24 could be induced by at least two stresses. Under cold stress, CsCBL9 and CsCIPK4/6a/6b/7/11/14b/19/20 were upregulated in both mature leaves and young shoots, CsCBL1/3/5 and CsCIPK1/8/10a/10b/10c/12/14a/23a/24 were induced only in mature leaves, and CsCIPK5/25 were induced only in young shoots. Yeast two-hybrid analysis showed that CsCBL1 could interact with CsCIPK1/10b/12 but not with CsCIPK6a/7/11/14b/20. CsCBL9 was found to interact with CsCIPK1/10b/12/14b but not with CsCIPK6a/7/11/20. These results suggest divergent responses to cold stress regulated by CBL-CIPK complexes between tea plant and Arabidopsis, as well as between mature leaves and young shoots in tea plant. A model of Ca-CsCBL-CsCIPK module-mediated abiotic stress signaling in tea plant is proposed.
钙调磷酸酶 B 类似蛋白（CBL）是一类钙结合蛋白，通过与 CBL 相互作用蛋白激酶（CIPK）特异性相互作用，在钙信号转导中发挥重要作用，这两个基因家族及其相互作用的复合物参与调节植物对各种环境刺激的反应。本研究在茶树中鉴定了 8 个 CBL 和 25 个 CIPK 基因，分别分为四个和五个亚家族。分析这些基因在非生物胁迫（成熟叶片冷处理、盐胁迫和 PEG 处理及幼梢冷处理）下的表达情况表明，CsCBL1/3/5 和 CsCIPK1/4/5/6a/7/8/10b/10c/12/14a/19/23a/24 可至少受两种胁迫诱导。在冷胁迫下，CsCBL9 和 CsCIPK4/6a/6b/7/11/14b/19/20 在成熟叶片和幼梢中均上调，CsCBL1/3/5 和 CsCIPK1/8/10a/10b/10c/12/14a/23a/24 仅在成熟叶片中诱导，而 CsCIPK5/25 仅在幼梢中诱导。酵母双杂交分析表明，CsCBL1 可与 CsCIPK1/10b/12 相互作用，但不能与 CsCIPK6a/7/11/14b/20 相互作用。CsCBL9 与 CsCIPK1/10b/12/14b 相互作用，但不能与 CsCIPK6a/7/11/20 相互作用。这些结果表明，茶树和拟南芥之间以及茶树成熟叶片和幼梢之间的 CBL-CIPK 复合物对冷胁迫的反应存在差异。提出了一个茶树中 Ca-CsCBL-CsCIPK 模块介导的非生物胁迫信号模型。
