Aesthet Surg J. 2021 Jan 1;41(1):86-97. doi: 10.1093/asj/sjaa170.
Fat processing plays a pivotal role in graft survival. Each component of the blood in lipoaspirate affects fat survival in different ways, but the mechanisms are not clear.
The aim of this study was to investigate, by various experimental methods, the effect of blood on the viability of fat grafts and adipose stem cells (ASCs).
Blood and fat samples were obtained from 6 female patients undergoing aesthetic liposuction. For the in vivo experiment, we compared fat mixed with normal saline or various ratios of blood in nude mice. The samples were explanted at 2 and 8 weeks to evaluate the gross volume retention and histologic and immunohistochemical characteristics. For in vitro experiments, ASCs were pretreated with hemoglobin at different concentrations and for different times. We then assessed the proliferation, migration, adipogenesis, and reactive oxygen species production of ASCs.
Blood in the graft led to a decrease in graft viability, as evaluated by general observation and histologic and immunohistochemical morphology in vivo. In vitro experiments showed inhibited proliferation, migration, and adipogenesis, and increased reactive oxygen species production in ACSs, after hemoglobin treatment, suggesting impaired ASC viability.
This study suggests that blood impairs the viability of fat grafts and ASCs and provides evidence that washing to remove blood is important in fat processing.
脂肪处理在移植物存活中起着关键作用。脂肪抽吸物中的血液的每个成分以不同的方式影响脂肪的存活，但机制尚不清楚。
本研究旨在通过各种实验方法研究血液对脂肪移植物和脂肪干细胞（ASCs）活力的影响。
从 6 名接受美容吸脂术的女性患者中获得血液和脂肪样本。对于体内实验，我们比较了脂肪与生理盐水或不同比例血液混合在裸鼠中的情况。在 2 周和 8 周时取出样本，评估大体体积保留和组织学及免疫组织化学特征。对于体外实验，将 ASCs 用不同浓度和不同时间的血红蛋白预处理。然后评估 ASCs 的增殖、迁移、成脂分化和活性氧物质的产生。
体内实验通过一般观察、组织学和免疫组织化学形态学评估，发现移植物中的血液导致移植物活力下降。体外实验表明，血红蛋白处理后 ASCs 的增殖、迁移和成脂分化受到抑制，活性氧物质产生增加，提示 ASCs 活力受损。
本研究表明血液会损害脂肪移植物和 ASCs 的活力，并提供了证据表明在脂肪处理过程中，清洗以去除血液很重要。
