Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, 1838 Guangzhou North Road, Guangzhou, 510515, Guangdong, People's Republic of China.
Aesthetic Plast Surg. 2023 Oct;47(5):2074-2083. doi: 10.1007/s00266-023-03383-2. Epub 2023 May 25.
Fat transplantation retention rate is individualized and unpredictable. The presence of blood components and oil droplets in the injected lipoaspirate increases inflammation and fibrosis in a dose-dependent manner, and is probably the key factor associated with poor retention.
This study describes a volumetric fat grafting strategy based on optimization of grafts via screening intact fat particles and absorbing free oil droplets and impurities.
Centrifuged fat components were analyzed by n-hexane leaching. A special device was applied to de-oil intact fat components and obtain ultra-condensed fat (UCF). UCF was evaluated by scanning electron microscopy, particle size analysis, and flow cytometric analysis. Histological and immunohistochemical changes were investigated in a nude mouse fat graft model over 90 days.
The lower 50% of centrifuged fat was concentrated to 40% of the original volume to obtain UCF. In UCF, the free oil droplet content was less than 10%, more than 80% of particles were larger than 1000 µm, and architecturally important fat components were present. The retention rate of UCF was significantly higher than that of Coleman fat on day 90 (57.5 ± 2.7% vs. 32.8 ± 2.5%, p < 0.001). Histological analysis detected small preadipocytes with multiple intracellular lipid droplets on day 3 in UCF grafts, indicative of early adipogenesis. Angiogenesis and macrophage infiltration were observed in UCF grafts soon after transplantation.
Adipose regeneration with UCF involves rapid macrophage infiltration and exit, resulting in angiogenesis and adipogenesis. UCF may serve as a lipofiller which is beneficial for fat regeneration.
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脂肪移植的保留率因人而异且难以预测。注射脂肪抽吸物中存在血液成分和油滴会导致炎症和纤维化呈剂量依赖性增加，这可能是与保留率差相关的关键因素。
本研究描述了一种基于优化移植物的容积脂肪移植策略，该策略通过筛选完整的脂肪颗粒和吸收游离油滴和杂质来实现。
通过正己烷浸提分析离心后的脂肪成分。应用一种特殊的装置对完整的脂肪成分进行除油，以获得超浓缩脂肪（UCF）。通过扫描电子显微镜、粒度分析和流式细胞术分析来评估 UCF。在裸鼠脂肪移植模型中，研究了 90 天内的组织学和免疫组织化学变化。
将离心后的下 50%的脂肪浓缩至原始体积的 40%以获得 UCF。在 UCF 中，游离油滴含量小于 10%，超过 80%的颗粒大于 1000μm，并且存在结构上重要的脂肪成分。UCF 的保留率在第 90 天显著高于 Coleman 脂肪（57.5±2.7% vs. 32.8±2.5%，p<0.001）。UCF 移植后第 3 天，组织学分析检测到具有多个细胞内脂质滴的小前脂肪细胞，表明早期脂肪生成。在 UCF 移植后不久，就观察到血管生成和巨噬细胞浸润。
UCF 脂肪再生涉及快速的巨噬细胞浸润和退出，导致血管生成和脂肪生成。UCF 可能作为一种脂肪填充物，有利于脂肪再生。
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