School of Veterinary Medicine, University College Dublin, Dublin, Ireland.
Telenostic Limited, Kilkenny, Ireland.
Parasitology. 2020 Sep;147(11):1249-1253. doi: 10.1017/S0031182020001031. Epub 2020 Jun 24.
New ideas for diagnostics in clinical parasitology are needed to overcome some of the difficulties experienced in the widespread adoption of detection methods for gastrointestinal parasites in livestock. Here we provide an initial evaluation of the performance of a newly developed automated device (Telenostic) to identify and quantify parasitic elements in fecal samples. This study compared the Telenostic device with the McMaster and Mini-FLOTAC for counting of strongyle eggs in a fecal sample. Three bovine fecal samples were examined, in triplicate, on each of the three fecal egg-counting devices. In addition, both manual (laboratory technician) and automated analysis (image analysis algorithm) were performed on the Telenostic device to calculate fecal egg counts (FEC). Overall, there were consistent egg counts reported across the three devices and calculation methods. The Telenostic device compared very favourably to the Mini-FLOTAC and McMaster. Only in sample C, a significant difference (P < 0.05) was observed between the egg counts obtained by Mini-FLOTAC and by the other methods. From this limited dataset it can be concluded that the Telenostic-automated test is comparable to currently used benchmark FEC methods, while improving the workflow, test turn-around time and not requiring trained laboratory personnel to operate or interpret the results.
需要新的临床寄生虫学诊断理念来克服在广泛采用家畜胃肠道寄生虫检测方法时遇到的一些困难。在这里,我们初步评估了一种新开发的自动化设备(Telenostic)在识别和定量粪便样本中寄生虫元素方面的性能。本研究比较了 Telenostic 设备与麦克马斯特(McMaster)和 Mini-FLOTAC 计数粪便样本中粗(Strongyle)虫卵的性能。在三种粪便虫卵计数设备上,每个样本重复检测 3 次。此外,对 Telenostic 设备进行了手动(实验室技术员)和自动化(图像分析算法)分析,以计算粪便虫卵计数(FEC)。总体而言,三种设备和计算方法报告的虫卵计数一致。Telenostic 设备与 Mini-FLOTAC 和 McMaster 比较非常有利。仅在样本 C 中,Mini-FLOTAC 与其他方法获得的虫卵计数存在显著差异(P < 0.05)。从这个有限的数据集可以得出结论,Telenostic 自动化测试与目前使用的基准 FEC 方法相当,同时改善了工作流程、测试周转时间,并且不需要经过培训的实验室人员来操作或解释结果。