Khillan J S, Deen K C, Yu S H, Sweet R W, Rosenberg M, Westphal H
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Nucleic Acids Res. 1988 Feb 25;16(4):1423-30. doi: 10.1093/nar/16.4.1423.
Transgenic mice were generated carrying either the long terminal repeat of Human Immunodeficiency Virus fused to the bacterial chloramphenicol acetyl transferase reporter gene or a control element of the murine alpha A crystallin gene fused to the tat gene of human immunodeficiency virus. By crossing these two strains, progeny were obtained which carried both transgenes. The bacterial reporter gene was specifically transactivated in the eyes of these animals.
通过基因工程技术培育出了转基因小鼠，这些小鼠携带了与细菌氯霉素乙酰转移酶报告基因融合的人类免疫缺陷病毒长末端重复序列，或者携带了与人类免疫缺陷病毒tat基因融合的小鼠αA晶体蛋白基因的控制元件。通过将这两个品系杂交，获得了同时携带这两个转基因的后代。在这些动物的眼睛中，细菌报告基因被特异性地反式激活。
