Yao Yuebao, Mi Wenjuan, Cao Guangzhao, Yang Ruiqi, Chen Huirong, Liu Yong, Zou Huiqin, Yan Yonghong
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
Institute of Chinese Medicine Research, Yangtze River Pharmaceutical Group Jiangsu Longfeng Tang Traditional Chinese Medicine Co., Ltd., Taizhou, China.
Front Pharmacol. 2020 Jun 5;11:711. doi: 10.3389/fphar.2020.00711. eCollection 2020.
is a famous traditional Chinese medicine (TCM) that can exert beneficial effects during the treatment of gastrointestinal diseases and is used widely in China and other countries in Southeast Asia. However, the nonvolatile active ingredients that are present in the water extractions from used to treat gastrointestinal diseases have yet to be elucidated. The goal of this study was to identify the nonvolatile active ingredients of .
We used an single-pass intestinal perfusion (SPIP) model to identify the active ingredients of that play significant roles in gastrointestinal absorption. In addition, we developed a high-performance liquid chromatography (HPLC) method to identify key fractions in intestinal outflow perfusate.
Nineteen components were identified in a water extraction from ; these exhibited different absorption capabilities in different intestinal segments. Of these, six components were determined by the newly developed HPLC method: catechin, vanillic acid, epicatechin, polydatin, isoquercitrin, and quercitrin.
The current study aimed to identify the active ingredients present in water extractions prepared from in a single-intestinal perfusate from rats. Our findings provide an experimental basis to explain the pharmacodynamic actions of .
是一种著名的传统中药，在胃肠道疾病治疗中可发挥有益作用，在中国及其他东南亚国家广泛应用。然而，用于治疗胃肠道疾病的水提取物中存在的非挥发性活性成分尚未阐明。本研究的目的是鉴定的非挥发性活性成分。
我们使用大鼠单通道肠道灌注（SPIP）模型来鉴定在胃肠道吸收中起重要作用的活性成分。此外，我们开发了一种高效液相色谱（HPLC）方法来鉴定肠道流出灌注液中的关键组分。
从的水提取物中鉴定出19种成分；这些成分在不同肠段表现出不同的吸收能力。其中，通过新开发的HPLC方法确定了6种成分：儿茶素、香草酸、表儿茶素、白藜芦醇、异槲皮苷和槲皮苷。
本研究旨在鉴定大鼠单肠灌注液中制备的水提取物中的活性成分。我们的研究结果为解释的药效作用提供了实验依据。
