Dell'Aquila Gianluca, Zauner Stefan, Heimerl Thomas, Kahnt Jörg, Samel-Gondesen Vera, Runge Simon, Hempel Franziska, Maier Uwe G
Laboratory for Cell Biology, Philipps University of Marburg, Marburg, Germany.
SYNMIKRO Research Center, Marburg, Germany.
Front Plant Sci. 2020 Jun 3;11:579. doi: 10.3389/fpls.2020.00579. eCollection 2020.
Unicellular organisms that live in marine environments must cope with considerable fluctuations in the availability of inorganic phosphate (P). Here, we investigated the extracellular P concentration-dependent expression, as well as the intracellular or extracellular localization, of phosphatases and phosphate transporters of the diatom . We identified P-regulated plasma membrane-localized, ER-localized, and secreted phosphatases, in addition to plasma membrane-localized, vacuolar membrane-localized, and plastid-surrounding membrane-localized phosphate transporters that were also regulated in a P concentration-dependent manner. These studies not only add further knowledge to already existing transcriptomic data, but also highlight the capacity of the diatom to distribute P intracellularly and to mobilize P from extracellular and intracellular resources.
生活在海洋环境中的单细胞生物必须应对无机磷（P）可用性的大幅波动。在此，我们研究了硅藻磷酸酶和磷酸盐转运蛋白的细胞外磷浓度依赖性表达以及细胞内或细胞外定位。我们鉴定出了受磷调节的质膜定位、内质网定位和分泌型磷酸酶，以及质膜定位、液泡膜定位和质体周围膜定位的磷酸盐转运蛋白，这些转运蛋白也以磷浓度依赖性方式受到调节。这些研究不仅为已有的转录组数据增添了更多知识，还突出了硅藻在细胞内分配磷以及从细胞外和细胞内资源中调动磷的能力。
