Suppr超能文献

新种,从西太平洋分离得到。

sp. nov., isolated from the West Pacific Ocean.

作者信息

Xue Zehao, Zhu Sidong, Chen Xiunuan, Chen Ting, Ren Na, Chen Yong, Dong Bingxia, Shen Xudong, Huang Yizhe, Yang Jifang, Chen Jigang

机构信息

College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, PR China.

Institute of Respiratory and Disease, Shenzhen People's Hospital, Shenzhen 518020, PR China.

出版信息

Int J Syst Evol Microbiol. 2020 Jul;70(7):4345-4350. doi: 10.1099/ijsem.0.004295.

Abstract

A Gram-stain-negative bacterium, designated strain 40Bstr34, isolated from a sediment sample from the West Pacific Ocean, was taxonomically characterized by using a polyphasic approach. The strain was phylogenetically close to LZB041 and CBS5Q-3, with 16S rRNA gene sequence similarities of 98.5 and 97.1 %, respectively. The genome of strain 40Bstr34 featured a G+C content of 65.7 % for a 5.8 Mb chromosome. Up-to-date bacterial core gene set analysis revealed that strain 40Bstr34 represents one independent lineage with LZB041. DNA-DNA hybridization values between strain 40Bstr34 and its phylogenetic neighbours ranged from 30.3-34.2 %, below the cutoff of 70 %. In addition, the corresponding average nucleotide identity values were between 81.8-83.7 %, which are lower than 95 % threshold. The predominant cellular fatty acids of strain 40Bstr34 were summed feature 8 (C ω and/or C ω), cyclo-C ω8 and iso-C 3-OH, and ubiquinone-10 as the predominant respiratory quinone. The major polar lipids included phosphatidylethanolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids and two unidentified lipids. Based on the results of phenotypic, chemotaxonomic and genetic analyses, strain 40Bstr34 is identified as representing a novel species of the genus for which the name sp. nov. is proposed. The type strain is 40Bstr34 (=JCM 33903=MCCC 1K04569).

摘要

从西太平洋沉积物样本中分离出的一株革兰氏阴性菌,命名为菌株40Bstr34,采用多相分类法对其进行了分类学特征分析。该菌株在系统发育上与LZB041和CBS5Q-3接近,16S rRNA基因序列相似性分别为98.5%和97.1%。菌株40Bstr34的基因组具有一个5.8 Mb染色体,G+C含量为65.7%。最新的细菌核心基因组分析表明,菌株40Bstr34与LZB041代表一个独立的谱系。菌株40Bstr34与其系统发育邻株之间的DNA-DNA杂交值在30.3%-34.2%之间,低于70%的阈值。此外,相应的平均核苷酸同一性值在81.8%-83.7%之间,低于95%的阈值。菌株40Bstr34的主要细胞脂肪酸为特征性总和8(C ω和/或C ω)、环C ω8和异C 3-OH,以泛醌-10作为主要呼吸醌。主要极性脂包括磷脂酰乙醇胺、磷脂酰单甲基乙醇胺、二磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、两种未鉴定的氨基脂和两种未鉴定的脂。基于表型、化学分类和遗传分析结果,菌株40Bstr34被鉴定为代表一个新的属种,为此提出新种名为sp. nov.。模式菌株为40Bstr34(=JCM 33903=MCCC 1K04569)。

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验