Life Sciences Institute, Zunyi Medical University, Zunyi, 563006, People's Republic of China.
College of Pharmacy, Hunan Vocational College of Science and Technology, Changsha, 410200, People's Republic of China.
Arch Microbiol. 2022 Nov 11;204(12):700. doi: 10.1007/s00203-022-03320-y.
A Gram-stain-negative and short rod-shaped strain CBK1P-4, isolated from surface-sterilized bark of Avicennia marina was investigated by a polyphasic taxonomic approach to resolve its taxonomic position. Strain CBK1P-4 grew at 10-30 °C (optimum, 25 °C), pH 5.0-9.0 (optimum, pH 5.5) and in the presence of 0-9% (w/v) NaCl (optimum, 1-2%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CBK1P-4 belonged to the genus Jiella and was most closely related to species of the genus Jiella (97.4-98.3%). The genome comparisons between strain CBK1P-4 and the closely related species indicated that average nucleotide identity and digital DNA-DNA hybridization values were below the recommended thresholds for assigning strains to the same species (95-96% and 70%, respectively). The cell wall peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The principal fatty acids were Cω7c and Ccycloω8c. The polar lipids were mainly comprised of phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, two unidentified aminolipids, one unidentified phospholipid and one unidentified glycolipid. The dominant respiratory quinone was ubiquinone-10. The DNA G + C content of strain CBK1P-4 was 66.7%. Based on the phenotypic features, phylogenetic analysis as well as genome analysis, we conclude that strain CBK1P-4 represents a novel Jiella species, for which the name Jiella avicenniae sp. nov. is proposed. The type strain is CBK1P-4 (= CGMCC 1.18742 = JCM 34330).
一株从海芒果表面消毒树皮中分离得到的短杆状革兰氏阴性菌株 CBK1P-4，通过多相分类学方法进行研究，以确定其分类地位。菌株 CBK1P-4 在 10-30°C（最适温度 25°C）、pH5.0-9.0（最适 pH5.5）和 0-9%（w/v）NaCl（最适 1-2%）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CBK1P-4 属于 Jiella 属，与 Jiella 属的种最为密切相关（97.4-98.3%）。菌株 CBK1P-4 与密切相关的种之间的基因组比较表明，平均核苷酸同一性和数字 DNA-DNA 杂交值均低于将菌株归为同一物种的推荐阈值（分别为 95-96%和 70%）。细胞壁肽聚糖含有 meso-二氨基庚二酸作为诊断二氨基酸。主要脂肪酸为 Cω7c 和 Ccycloω8c。极性脂主要由磷脂酰胆碱、双磷脂酰甘油、磷脂酰甘油、磷脂酰单甲乙醇胺、磷脂酰乙醇胺、两种未鉴定的胺脂、一种未鉴定的磷脂和一种未鉴定的糖脂组成。主要呼吸醌为泛醌-10。菌株 CBK1P-4 的 DNA G+C 含量为 66.7%。基于表型特征、系统发育分析以及基因组分析，我们得出结论，菌株 CBK1P-4 代表了一个新的 Jiella 种，建议将其命名为 Jiella avicenniae sp. nov.。模式菌株为 CBK1P-4（= CGMCC 1.18742= JCM 34330）。
