Microbial Oceanography Laboratory, School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, Seoul 08826, Republic of Korea.
Saemangeum Environmental Research Center, Kunsan National University, Kunsan 54150, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.006139.
A novel Gram-stain-negative, aerobic, rod-shaped bacterium, designated as HL-NP1, was isolated from the surface water of the northwestern Pacific Ocean after enrichment cultivation using the organic phosphorous compound of 2-aminoethylphosphonate. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus , with the highest similarity to 40Bstr34 (98.7 %). The complete genome sequence of strain HL-NP1 comprised a circular chromosome of 5.58 Mbp and two circular plasmids of 0.15 and 0.22 Mbp. Comparison of the genome sequences between strains HL-NP1 and 40Bstr34 revealed that average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values (88.0, 86.4 and 33.9 %, respectively) were below the recommended cut-off levels for delineating bacterial species. Strain HL-NP1 showed optimal growth at 30 °C, pH 6.5-7.0, with 2.0-2.5 % (w/v) NaCl. The sole respiratory quinone was ubiquinone-10. The predominant fatty acid was summed feature 8 (C 6 and/or C 7). The polar lipids comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, an unidentified aminolipid and four unidentified lipids. The G+C content of the genomic DNA was 65.1 %. Based on phylogenetic, genotypic, phenotypic and chemotaxonomic data, strain HL-NP1 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HL-NP1 (= KCCM 90499 = JCM 35838).
一株新型革兰氏阴性、需氧、杆状细菌，命名为 HL-NP1，是通过使用 2-氨基乙基膦酸盐这种有机磷化合物对西北太平洋的地表水进行富集培养后分离得到的。基于 16S rRNA 基因序列的系统发育分析表明，该菌株属于 属，与 40Bstr34（98.7%）的相似度最高。菌株 HL-NP1 的全基因组序列由一个 5.58 Mbp 的环状染色体和两个 0.15 和 0.22 Mbp 的环状质粒组成。HL-NP1 菌株和 40Bstr34 菌株的基因组序列比较表明，平均核苷酸同一性、平均氨基酸同一性和数字 DNA-DNA 杂交值（分别为 88.0%、86.4%和 33.9%）均低于用于划定细菌种的推荐截止值。HL-NP1 菌株在 30°C、pH6.5-7.0、2.0-2.5%（w/v）NaCl 条件下生长最佳。唯一的呼吸醌是泛醌-10。主要脂肪酸是 sum feature 8（C 6 和/或 C 7）。极性脂包括双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰单甲基乙醇胺、一种未鉴定的胺脂和四种未鉴定的脂类。基因组 DNA 的 G+C 含量为 65.1%。基于系统发育、基因型、表型和化学分类学数据，HL-NP1 菌株被提议代表一个属的新型种，建议命名为 sp. nov.。模式菌株为 HL-NP1（=KCCM 90499=JCM 35838）。
