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金纳米粒子作为比色传感器用于检测 DNA 碱基及其相关化合物。

Gold Nanoparticles as Colorimetric Sensors for the Detection of DNA Bases and Related Compounds.

机构信息

Departamento de Química. Facultad de Ciencias. Campus A Zapateira, Universidade A Coruña, 15008-La Coruña, Spain.

出版信息

Molecules. 2020 Jun 23;25(12):2890. doi: 10.3390/molecules25122890.

DOI:10.3390/molecules25122890
PMID:32586064
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7356728/
Abstract

Results regarding interaction of colloidal gold solutions with nucleobases, including uracil (U), as well as its sulfur derivatives, 2-thiouracil (2TU) and 4-thiouracil (4TU), cytosine (C), adenine (A), and guanine (G), as well as urea and thiourea (TU), are reported. Anionic stabilized citrate gold nanoparticles (AuNPs) were synthesized by reducing the tetrachloroaurate (III) trihydrate with trisodium citrate. The surface plasmon resonance (SPR) band was used in the characterization of synthesized AuNPs, as well as transmission electron microscope (TEM) imaging, which was used in the characterization of dispersed and aggregated gold nanoparticles. Interactions of nucleobases with the gold surface was analyzed by following the plasmon absorbance band red shift of the AuNPs. The sulfur-containing compounds adsorbed to the nanoparticle surfaces by chemisorption-type interactions; with TU and 4TU, the process is accompanied by a sudden change in color; in contrast, 2TU forms stable functionalized gold nanoparticles. Urea and U do not adsorb to nanoparticle surfaces, but the other heterocyclic bases containing nitrogen interact effectively with the gold surface, causing the assembly of nanoparticles, even though the interparticle self-aggregation process was slower than that mediated by either TU or 4TU. The method is efficient in the colorimetric detection of nucleobases and derivatives at concentration levels on the order of 1 µM.

摘要

报告了胶体金溶液与核苷碱基（包括尿嘧啶（U）及其硫代衍生物 2-硫代尿嘧啶（2TU）和 4-硫代尿嘧啶（4TU）、胞嘧啶（C）、腺嘌呤（A）和鸟嘌呤（G））以及脲和硫脲（TU）相互作用的结果。通过用三钠柠檬酸盐还原三氯金酸三水合物合成了带负电荷的稳定柠檬酸金纳米粒子（AuNPs）。表面等离子体共振（SPR）带用于合成 AuNPs 的表征，以及透射电子显微镜（TEM）成像，用于分散和聚集金纳米粒子的表征。通过观察 AuNPs 的等离子体吸收带的红移分析了碱基与金表面的相互作用。含硫化合物通过化学吸附型相互作用吸附到纳米粒子表面；对于 TU 和 4TU，该过程伴随着颜色的突然变化；相比之下，2TU 形成稳定的功能化金纳米粒子。脲和 U 不吸附到纳米粒子表面，但其他含氮的杂环碱基与金表面有效相互作用，导致纳米粒子的组装，尽管粒子间的自组装过程比 TU 或 4TU 介导的过程要慢。该方法在检测浓度为 1µM 左右的碱基和衍生物的比色法中非常有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/2628b9c7f2ea/molecules-25-02890-sch003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/b0b655044c9a/molecules-25-02890-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/1aa0675d56b9/molecules-25-02890-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/7f2ec33fdf02/molecules-25-02890-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/29f928d879fd/molecules-25-02890-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/fc3e068d6f09/molecules-25-02890-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/821c8399bf3e/molecules-25-02890-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/2ab1fd8a6013/molecules-25-02890-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/7768e04c0db2/molecules-25-02890-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/2628b9c7f2ea/molecules-25-02890-sch003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/b0b655044c9a/molecules-25-02890-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/1aa0675d56b9/molecules-25-02890-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/7f2ec33fdf02/molecules-25-02890-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/29f928d879fd/molecules-25-02890-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/fc3e068d6f09/molecules-25-02890-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/821c8399bf3e/molecules-25-02890-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/2ab1fd8a6013/molecules-25-02890-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/7768e04c0db2/molecules-25-02890-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0b2/7356728/2628b9c7f2ea/molecules-25-02890-sch003.jpg

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