Cherng Juin-Hong, Lin Cheng-An J, Liu Cheng-Che, Yeh Jue-Zong, Fan Gang-Yi, Tsai Hsin-Da, Chung Chun-Fang, Hsu Sheng-Der
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan.
Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, Taipei 114, Taiwan.
J Pers Med. 2022 Jun 30;12(7):1089. doi: 10.3390/jpm12071089.
Burn injuries are a common hazard in the military, as fire is likely to be weaponized. Thus, it is important to find an effective substance to accelerate burn wound healing. This study used chitosan and gold nanoparticles (AuNPs) as wound dressings and investigated their effectiveness in femoral artery hemorrhage swine and rat burn models. Chitosan dressing has significant hemostatic properties compared with gauze. Histological results showed that burn wounds treated with chitosan or AuNP-coated chitosan dressings exhibited more cells and a continuous structure of the epidermis and dermis than those of the control and untreated lesion groups. Furthermore, both chitosan dressings have been shown to positively regulate the expression of genes- and cytokines/chemokines-related to the wound healing process; AuNP-coated chitosan significantly lessened severe sepsis and inflammation, balanced the activities of pro-fibrotic and anti-fibrotic ligands for tissue homeostasis, regulated angiogenesis, and inhibited apoptosis activity, thereby being beneficial for the burn microenvironment. Hence, chitosan alone or in combination with AuNPs represents a prospective therapeutic substance as a burn dressing which might be helpful for burn wound care. This study provides a novel hemostasis dressing for modern warfare that is simple to use by most medical and paramedical personnel handling for burn treatment.
烧伤是军事行动中的常见危险，因为火很可能被用作武器。因此，找到一种有效的物质来加速烧伤创面愈合非常重要。本研究使用壳聚糖和金纳米颗粒（AuNPs）作为伤口敷料，并在股动脉出血猪和大鼠烧伤模型中研究了它们的有效性。与纱布相比，壳聚糖敷料具有显著的止血性能。组织学结果表明，与对照组和未处理病变组相比，用壳聚糖或金纳米颗粒包被的壳聚糖敷料处理的烧伤创面显示出更多的细胞以及连续的表皮和真皮结构。此外，两种壳聚糖敷料均已显示出对与伤口愈合过程相关的基因以及细胞因子/趋化因子的表达具有正向调节作用；金纳米颗粒包被的壳聚糖显著减轻了严重脓毒症和炎症，平衡了促纤维化和抗纤维化配体的活性以维持组织稳态，调节血管生成，并抑制细胞凋亡活性，从而有利于烧伤微环境。因此，单独使用壳聚糖或与金纳米颗粒联合使用代表了一种有前景的治疗物质，可作为烧伤敷料，这可能有助于烧伤创面护理。本研究为现代战争提供了一种新型止血敷料，大多数处理烧伤治疗的医护人员都易于使用。
