Department of Radiotherapy, Taizhou Center Hospital, Taizhou City, Zhejiang Province, China.
Department of Respiratory Medicine, Taizhou First People's Hospital, Taizhou City, Zhejiang Province, China.
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820922558. doi: 10.1177/1533033820922558.
MicroRNAs have been demonstrated to be critical regulators in tumor progression, including non-small cell lung cancer. MicroRNA-222-3p has been reported to function as a tumor suppressor or oncogene in several types of cancer, but its function role in non-small cell lung cancer has not been uncovered. In this study, we first found the expression of microRNA-222-3p was significantly increased in non-small cell lung cancer tissues and cell lines. MicroRNA-222-3p inhibitor decreased the activity of non-small cell lung cancer cells to proliferate and increased cell apoptosis using cell counting kit-8, flow cytometry, and caspase-3 activity analysis. Overexpressed microRNA-222-3p in non-small cell lung cancer cells promoted cell proliferation, but decreased cell apoptosis. Moreover, Bcl-2-binding component 3 was the target gene of microRNA-222-3p, and its knockdown weakened the regulatory effect of microRNA-222-3p inhibitor on cell proliferation and apoptosis in non-small cell lung cancer cells. In conclusion, microRNA-222-3p plays a significant role in the regulation of Bcl-2-binding component 3 expression and might be a promising target for clinical non-small cell lung cancer therapy.
MicroRNAs 已被证明在肿瘤进展中起着关键的调节作用，包括非小细胞肺癌。MicroRNA-222-3p 已被报道在几种类型的癌症中作为肿瘤抑制因子或癌基因发挥作用，但它在非小细胞肺癌中的功能作用尚未被揭示。在这项研究中，我们首先发现 MicroRNA-222-3p 的表达在非小细胞肺癌组织和细胞系中显著增加。使用细胞计数试剂盒-8、流式细胞术和 caspase-3 活性分析，MicroRNA-222-3p 抑制剂降低了非小细胞肺癌细胞的活性，抑制了细胞增殖并增加了细胞凋亡。在非小细胞肺癌细胞中过表达 MicroRNA-222-3p 促进了细胞增殖，但降低了细胞凋亡。此外，Bcl-2 结合成分 3 是 MicroRNA-222-3p 的靶基因，其敲低削弱了 MicroRNA-222-3p 抑制剂对非小细胞肺癌细胞增殖和凋亡的调节作用。总之，MicroRNA-222-3p 在调节 Bcl-2 结合成分 3 的表达中起着重要作用，可能是临床非小细胞肺癌治疗的有前途的靶点。
