Zhang Xinai, Zhu Minchen, Jiang Yanjuan, Wang Xin, Guo Zhiming, Shi Jiyong, Zou Xiaobo, Han En
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
J Hazard Mater. 2020 Dec 5;400:123222. doi: 10.1016/j.jhazmat.2020.123222. Epub 2020 Jun 17.
A convenient sensor is developed for electrochemical assay of Hg in dairy product using the optimal Cu-based metal-organic frameworks (Cu-MOFs) as signal reporting. Benefiting from specific recognition between Hg and thymine (T)-rich DNA strands, the interferences of milk matrices are effectively eliminated, thereby greatly improving the accuracy of test results. Moreover, the suitable Cu-MOFs offer an efficient carrier for probe design, and the contained Cu ions could be directly detected to output electrochemical signal of Hg presence without labor- or time-intensive operations. Compared with previous methods, this sensor substantially simplifies the process of electrochemical measurement and facilitates highly sensitive, selective and rapid analysis of Hg with detection limit of 4.8 fM, offering a valuable means for monitoring dairy product contamination with Hg.
开发了一种便捷的传感器，用于以最佳的铜基金属有机框架（Cu-MOFs）作为信号报告物对乳制品中的汞进行电化学检测。受益于汞与富含胸腺嘧啶（T）的DNA链之间的特异性识别，有效地消除了牛奶基质的干扰，从而大大提高了测试结果的准确性。此外，合适的Cu-MOFs为探针设计提供了有效的载体，并且所含的铜离子可以直接被检测以输出汞存在的电化学信号，而无需耗费大量人力或时间的操作。与以前的方法相比，该传感器大大简化了电化学测量过程，并有助于对汞进行高灵敏度、高选择性和快速分析，检测限为4.8 fM，为监测乳制品中的汞污染提供了一种有价值的手段。
