Kunamneni Adinarayana, Ogaugwu Christian, Bradfute Steven, Durvasula Ravi
Department of Medicine, Loyola University Medical Center, Chicago, IL 60153, USA.
Department of Animal and Environmental Biology, Federal University Oye-Ekiti, Ekiti State 371010, Nigeria.
Antibodies (Basel). 2020 Jun 27;9(3):28. doi: 10.3390/antib9030028.
Antibody ribosome display remains one of the most successful in vitro selection technologies for antibodies fifteen years after it was developed. The unique possibility of direct generation of whole proteins, particularly single-chain antibody fragments (scFvs), has facilitated the establishment of this technology as one of the foremost antibody production methods. Ribosome display has become a vital tool for efficient and low-cost production of antibodies for diagnostics due to its advantageous ability to screen large libraries and generate binders of high affinity. The remarkable flexibility of this method enables its applicability to various platforms. This review focuses on the applications of ribosome display technology in biomedical and agricultural fields in the generation of recombinant scFvs for disease diagnostics and control.
抗体核糖体展示技术自开发十五年以来，仍然是最成功的抗体体外筛选技术之一。直接生成完整蛋白质，特别是单链抗体片段（scFv）的独特可能性，促使该技术成为最重要的抗体生产方法之一。核糖体展示因其具有筛选大型文库和产生高亲和力结合物的优势能力，已成为用于诊断的高效低成本抗体生产的重要工具。该方法具有显著的灵活性，使其适用于各种平台。本综述重点关注核糖体展示技术在生物医学和农业领域的应用，即在疾病诊断和控制中生成重组单链抗体片段。
