Warrington R J
Rheumatic Disease Unit Research Laboratory, University of Manitoba, Winnipeg, Canada.
J Rheumatol. 1988 Apr;15(4):616-20.
Interleukin-2 (IL-2) production in systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) was assessed at varying cell densities from 3 X 10(3)-1 X 10(5) cells/culture in response to mitogen stimulation, using a cellular interleukin assay in which IL-2 responsive CTLL-2 indicator cells were added directly to stimulated peripheral blood lymphocyte cultures. It was found that in both RA and SLE there was a significant overproduction of IL-2 demonstrable at low cell densities. At high cell densities, IL-2 production was decreased in SLE but remained enhanced in RA. The former abnormality appears to be related to disease activity and was not reversed by irradiation, suggesting that it is not the result of the actions of a radiation sensitive suppressor cell population. The possibility is discussed that a local hyper-production of IL-2 could induce disturbances of the immune response, resulting in the breaking of normal tolerance.
采用细胞白细胞介素检测法，即直接将白细胞介素-2（IL-2）反应性CTLL-2指示细胞添加到经丝裂原刺激的外周血淋巴细胞培养物中，在3×10³-1×10⁵个细胞/培养物的不同细胞密度下，评估系统性红斑狼疮（SLE）和类风湿性关节炎（RA）中IL-2的产生情况。结果发现，在RA和SLE中，在低细胞密度下均有明显的IL-2过量产生。在高细胞密度下，SLE中IL-2的产生减少，但RA中仍增强。前一种异常似乎与疾病活动有关，且不受辐射影响，这表明它不是辐射敏感抑制细胞群作用的结果。文中讨论了局部IL-2过度产生可能诱导免疫反应紊乱，导致正常耐受性破坏的可能性。
