Warrington R J, Sauder P J, Homik J, Ofosu-Appiah W
Rheumatic Disease Unit Research Laboratory, University of Manitoba, Winnipeg, Canada.
Clin Exp Immunol. 1989 Aug;77(2):163-7.
Limiting dilution analysis techniques were used to determine precursor frequencies for interleukin-2 (IL-2) responsive cells among the peripheral blood lymphocytes of patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis compared with healthy subjects. Response defects in SLE were found, but were of two types: reduced precursor frequencies with normal pattern of response (single-hit kinetics); and abnormal multi-hit responsiveness. These abnormalities were not more frequent statistically in those with active disease. Precursor frequencies of SLE peripheral blood lymphocytes were enhanced by resting the cells for up to 72 h prior to activation, and by adding exogenous IL-2 during the initial activation step. The IL-2 response defects of SLE are therefore reversible and may in part be secondary to other in vivo abnormalities, such as deficient IL-2 production.
采用有限稀释分析技术，测定了系统性红斑狼疮（SLE）和类风湿性关节炎患者外周血淋巴细胞中白细胞介素-2（IL-2）反应性细胞的前体细胞频率，并与健康受试者进行了比较。在SLE中发现了反应缺陷，但有两种类型：前体细胞频率降低但反应模式正常（单打击动力学）；以及异常的多打击反应性。这些异常在统计学上在活动性疾病患者中并不更常见。在激活前让SLE外周血淋巴细胞静置长达72小时，并在初始激活步骤中添加外源性IL-2，可提高其前体细胞频率。因此，SLE的IL-2反应缺陷是可逆的，并且可能部分继发于其他体内异常，如IL-2产生不足。
