Al-Judaibi Awatif A
Department of Biological Sciences-Microbiology section, Faculty of Science, Jeddah University/King Abdulaziz University, Jeddah, Saudi Arabia, P.O. Box: 13520, Jeddah 21414, Saudi Arabia.
AIMS Microbiol. 2020 May 18;6(2):121-143. doi: 10.3934/microbiol.2020008. eCollection 2020.
Despite the harsh conditions and limited water resources of the Arabian Peninsula, plants that live in this environment contain a variety of bioactive compounds and have been used in traditional medicines for thousands of years. We investigated the effects of ethanol extracts of and on Gram-negative and Gram-positive bacteria. The investigations were include; the inhibition of the bacterial growth, determination of MIC and MBC, detection of kill-time, potassium and phosphorus leakages and detection of the bioactive compounds by the GC-MS analysis. The tested extracts in combination, at a 1:1 volume ratio, showed high inhibitory effects, as reflected by the minimum inhibitory concentrations and minimum bactericidal concentrations. The new EC plate was used to determined MBC and kill-time. Further, the detection of phosphate and potassium leakage indicated a loss of selective permeability of the cytoplasmic membrane after treatment with these extracts. The bioactive compounds in the ethanol extracts of and may offer a less expensive and natural alternative to pharmaceuticals.
尽管阿拉伯半岛环境恶劣、水资源有限,但生活在该环境中的植物含有多种生物活性化合物,并且数千年来一直被用于传统医学。我们研究了[植物名称1]和[植物名称2]乙醇提取物对革兰氏阴性菌和革兰氏阳性菌的影响。研究内容包括:抑制细菌生长、测定最低抑菌浓度(MIC)和最低杀菌浓度(MBC)、检测杀菌时间、钾和磷泄漏情况以及通过气相色谱-质谱联用(GC-MS)分析检测生物活性化合物。以1:1体积比混合的受试提取物表现出高抑制作用,这通过最低抑菌浓度和最低杀菌浓度得以体现。使用新型EC平板来测定MBC和杀菌时间。此外,磷酸盐和钾泄漏检测表明,用这些提取物处理后,细胞质膜的选择性通透性丧失。[植物名称1]和[植物名称2]乙醇提取物中的生物活性化合物可能为药物提供一种成本更低的天然替代品。