Qingdao University, Qingdao, 266071, China.
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
Virol Sin. 2021 Feb;36(1):33-42. doi: 10.1007/s12250-020-00239-w. Epub 2020 Jul 2.
Zika virus (ZIKV) has been isolated from mosquitoes such as Aedes, Mansonia uniformis, and Culex perfuscus; However, the isolation of ZIKV from Anopheles sinensis and Culex tritaeniorhynchus has not yet been reported. In June and July 2018, 22,985 mosquitoes and 57,500 midges were collected in Jiangxi Province in southeastern China. Among them, six strains of ZIKV were isolated from mosquitoes: four from An. sinensis and two from Cx. tritaeniorhynchus. Molecular genetic analysis showed that the ZIKV isolated from An. sinensis and Cx. tritaeniorhynchus belonged to genotype 2 in the Asian evolutionary branch of ZIKV. In addition, the ZIKV strains isolated from An. sinensis and Cx. tritaeniorhynchus had amino acid substitutions identical to ZIKV strains prevalent in South America since 2015. This study is the first to isolate ZIKV from mosquito specimens collected in the wild of Jiangxi Province, China; This is also the first time that ZIKV has been isolated from An. sinensis and Cx. tritaeniorhynchus. Given that An. sinensis and Cx. tritaeniorhynchus have a very wide geographical distribution in China and even in eastern and southern Asia, the isolation of several strains of ZIKV from these two mosquitoes poses new challenges for the prevention and control of ZIKV infection in the mainland of China and countries and regions with the same distribution of mosquitoes.
寨卡病毒（ZIKV）已从蚊子中分离出来，如埃及伊蚊、曼蚊和库蚊；然而，尚未报道从中华按蚊和三带喙库蚊中分离出寨卡病毒。2018 年 6 月至 7 月，在中国东南部的江西省采集了 22985 只蚊子和 57500 只蠓。其中，从蚊子中分离出六株寨卡病毒：四株来自中华按蚊，两株来自三带喙库蚊。分子遗传分析表明，从中华按蚊和三带喙库蚊中分离出的寨卡病毒属于寨卡病毒亚洲进化枝中的基因型 2。此外，从中华按蚊和三带喙库蚊中分离出的寨卡病毒株与 2015 年以来在南美洲流行的寨卡病毒株具有相同的氨基酸取代。本研究首次从中国江西省野外采集的蚊子标本中分离出寨卡病毒；这也是首次从中华按蚊和三带喙库蚊中分离出寨卡病毒。鉴于中华按蚊和三带喙库蚊在中国乃至东亚和南亚的地理分布非常广泛，从这两种蚊子中分离出几株寨卡病毒，给中国大陆和具有相同蚊种分布的国家和地区寨卡病毒感染的防控带来了新的挑战。
