Civzele Anna, Stipniece-Jekimova Alise Anna, Mezule Linda
Water Systems and Biotechnology Institute, Riga Technical University, Riga, Latvia.
Fungal Biol Biotechnol. 2025 May 14;12(1):7. doi: 10.1186/s40694-025-00198-5.
Wastewater treatment plants (WWTPs), particularly activated sludge systems, generate significant amounts of various types of waste, including screenings, primary sludge, and secondary sludge. While substantial research has been conducted on the recovery and valorization of sewage sludge, the treatment and utilization of screenings remain underexplored. In response, this study investigates the potential of white rot fungi to degrade cellulose-containing waste screened during the preliminary treatment and examines the production patterns of lignocellulolytic enzymes in the presence of this waste. The studied fungi exhibited variable enzymatic responses depending on the type of substrate, however, their adaptability highlighted the potential in fungal-mediated bioconversion processes. P. dryinus and T. versicolor were identified as strong and adaptive candidates for oxidative enzyme production, with P. dryinus showing laccase activity up to 1691.75 ± 12.22 U/mg and degrading 44.46% of carbohydrates in tested screenings. I. lacteus and B. adusta were predominantly observed in cellulolytic enzyme production, with B. adusta ensuring a 43.49% reduction in carbohydrate content of screenings. As a result of fungal cultivation in WWTP waste, the production potential of 34 to 46 kg of sugars per ton of screenings was determined. Therefore, the study presents a promising approach for the sustainable treatment of screenings and the development of waste management and resource recovery strategies for WWTP-derived waste.
污水处理厂（WWTPs），尤其是活性污泥系统，会产生大量各种类型的废物，包括筛余物、初沉污泥和二沉污泥。虽然对污水污泥的回收和增值利用已经进行了大量研究，但筛余物的处理和利用仍未得到充分探索。作为回应，本研究调查了白腐真菌降解预处理过程中筛选出的含纤维素废物的潜力，并研究了在这种废物存在下木质纤维素酶的产生模式。所研究的真菌根据底物类型表现出不同的酶促反应，然而，它们的适应性突出了真菌介导的生物转化过程的潜力。干酪皮孔菌和云芝被确定为氧化酶产生的强大且适应性强的候选菌株，干酪皮孔菌的漆酶活性高达1691.75±12.22 U/mg，并降解了测试筛余物中44.46%的碳水化合物。乳白耙齿菌和毛栓菌主要参与纤维素酶的产生，毛栓菌使筛余物中的碳水化合物含量降低了43.49%。通过在污水处理厂废物中培养真菌，确定了每吨筛余物产生34至46千克糖的生产潜力。因此，该研究为筛余物的可持续处理以及污水处理厂衍生废物的废物管理和资源回收策略的开发提供了一种有前景的方法。
