Manickasundari M, Pitchappan R
Department of Immunology, School of Biological Sciences, Madurai Kamaraj University, India.
Dev Comp Immunol. 1988 Summer;12(3):603-10. doi: 10.1016/0145-305x(88)90076-6.
Structural and functional characteristics of the lymphoid cells of Calotes versicolor were studied using thymocytes and splenocytes isolated on a buoyant density gradient and adherence column. The isolated cells were treated with anti-Calotes thymocytes serum (ATS) and their residual capacity to effect antigen specific migration inhibition and antibody production assessed. Results revealed that antigen specific migration inhibition effector cells could be enriched in less dense nylon wool-adherent fraction and 95% of this fraction were sensitive to ATS. Antigen specific plaque forming cells (PFC) could also be enriched in the same fraction but they were not susceptible to ATS.
利用在浮力密度梯度和黏附柱上分离得到的胸腺细胞和脾细胞，研究了变色树蜥淋巴细胞的结构和功能特征。将分离出的细胞用抗变色树蜥胸腺细胞血清（ATS）处理，并评估其影响抗原特异性迁移抑制和抗体产生的剩余能力。结果显示，抗原特异性迁移抑制效应细胞可在密度较低的尼龙毛黏附部分富集，该部分95%的细胞对ATS敏感。抗原特异性斑块形成细胞（PFC）也可在同一部分富集，但它们对ATS不敏感。
