Bampidis Vasileios, Azimonti Giovanna, Bastos Maria de Lourdes, Christensen Henrik, Dusemund Birgit, Kouba Maryline, Kos Durjava Mojca, López-Alonso Marta, López Puente Secundino, Marcon Francesca, Mayo Baltasar, Pechová Alena, Petkova Mariana, Ramos Fernando, Sanz Yolanda, Villa Roberto Edoardo, Woutersen Ruud, Aquilina Gabriele, Bories Georges, Chesson Andrew, Nebbia Carlo, Renshaw Derek, Innocenti Matteo Lorenzo, Gropp Jürgen
EFSA J. 2019 Jan 17;17(1):e05528. doi: 10.2903/j.efsa.2019.5528. eCollection 2019 Jan.
The additive cassia gum consists mainly of high-molecular weight polysaccharides composed primarily of a linear chain of 1,4-β-d-mannopyranose units with 1,6-linked α-d-galactopyranose units. In 2014, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) delivered an opinion on the safety and efficacy of cassia gum in cats and dogs. The Panel concluded, based on positive findings observed in a bacterial reverse mutation test with a semi-refined cassia gum (about 70 mg anthraquinones/kg) but not with purified semi-refined cassia gum that meets the specification as a food additive (< 0.5 mg anthraquinones/kg), that only purified semi-refined cassia gum that meets the specifications of cassia gum as a food additive can be considered safe for cats and dogs, at a maximum content of 1.5% cassia gum (15,000 mg/kg feed) in dry matter, corresponding to 1.32% (13,200 mg/kg feed) in a standardised complete feed with 12% water content. The conclusion was confirmed in an opinion delivered by the Panel in 2017. Following this opinion, the European Commission gave the possibility to the applicant to submit complementary information on the safety for cats and dogs. The semi-refined cassia gum under application was mutagenic in a bacterial reverse mutation test in Typhimurium strain TA100. Positive results were also observed after heat sterilisation of the test item. Isopropanol purified semi-refined cassia gum did not show mutagenic effect. Examination of various other cassia gum extracts did not identify any source of mutagenicity. The mutagenicity of the semi-refined cassia gum under application cannot be excluded. Therefore, the FEEDAP Panel cannot establish the safety of semi-refined cassia gum for cats and dogs.
添加型决明胶主要由高分子量多糖组成，这些多糖主要由1,4-β-D-甘露吡喃糖单元的线性链与1,6-连接的α-D-半乳吡喃糖单元构成。2014年，动物饲料添加剂和产品或物质专家委员会（FEEDAP）就决明胶在猫和狗中的安全性和有效性发表了意见。专家委员会得出结论，基于在使用半精制决明胶（约70毫克蒽醌/千克）进行的细菌回复突变试验中观察到的阳性结果，但在符合食品添加剂规格（<0.5毫克蒽醌/千克）的纯化半精制决明胶试验中未观察到阳性结果，只有符合决明胶食品添加剂规格的纯化半精制决明胶在干物质中决明胶最大含量为1.5%（15,000毫克/千克饲料）时，才被认为对猫和狗是安全的，这相当于在水分含量为12%的标准化全价饲料中为1.32%（13,200毫克/千克饲料）。该结论在专家委员会2017年发表的意见中得到了确认。根据该意见，欧盟委员会允许申请人提交关于猫和狗安全性的补充信息。所申请的半精制决明胶在鼠伤寒沙门氏菌TA100菌株的细菌回复突变试验中具有致突变性。对试验项目进行热灭菌后也观察到了阳性结果。异丙醇纯化的半精制决明胶未显示致突变作用。对各种其他决明胶提取物的检测未发现任何致突变源。所申请的半精制决明胶的致突变性不能排除。因此，FEEDAP专家委员会无法确定半精制决明胶对猫和狗的安全性。
