School of Psychological and Cognitive Sciences and Beijing Key Laboratory of Behavior and Mental Health, Peking University, Beijing, China.
Bioinformatics program, Boston University, Boston, MA, USA.
J Sleep Res. 2021 Feb;30(1):e13133. doi: 10.1111/jsr.13133. Epub 2020 Jul 7.
Job stress and the Circadian Locomotor Output Cycles Kaput (CLOCK) gene could affect circadian rhythm and sleep quality. The main aim of our present study was to investigate the association of job stress, CLOCK gene polymorphism and their interaction with sleep quality in a non-clinical Chinese Han population, which has not been reported to date. Using a cross-sectional design, 450 subjects were recruited in Beijing. Sleep quality was measured with the Pittsburgh Sleep Quality Index (PSQI) and job stress was measured with the Work Stress Scale. CLOCK gene rs11932595 polymorphism was genotyped in 297 blood samples. Correlation analysis showed a close but different association of high job stress with the PSQI and its components. Analysis of variance showed significant main effects of the CLOCK gene rs11932595 polymorphism. G-allele carriers had a higher score in the PSQI, sleep duration, sleep latency and sleep disturbances. Further interaction analyses showed an ordinal interaction on sleep duration, and a disordinal interaction on daytime dysfunction. Specifically, G-allele carriers had poorer sleep duration than AA homozygotes when in high job stress, while the two subgroups displayed similar sleep duration when in low job stress, conforming to the diathesis-stress model. In comparison to G-allele carriers, AA homozygotes experienced less daytime dysfunction when in low job stress whereas more daytime dysfunction when in high job stress, fitting with the differential susceptibility model. As genetic links have been revealed, our investigation might be conducive for elucidating aetiological factors for sleep quality and targets for implementing interventions to attain good sleep quality.
工作压力和生物钟输出周期基因（CLOCK）可能会影响昼夜节律和睡眠质量。我们目前的主要研究目的是调查工作压力、CLOCK 基因多态性及其与睡眠质量之间的相互作用，这在非临床汉族人群中尚未有报道。采用横断面设计，在北京招募了 450 名受试者。使用匹兹堡睡眠质量指数（PSQI）评估睡眠质量，使用工作压力量表评估工作压力。在 297 份血液样本中检测 CLOCK 基因 rs11932595 多态性。相关性分析显示，高工作压力与 PSQI 及其各成分密切相关，但存在差异。方差分析显示 CLOCK 基因 rs11932595 多态性存在显著的主要效应。G 等位基因携带者 PSQI、睡眠持续时间、入睡潜伏期和睡眠障碍评分较高。进一步的交互分析显示，在睡眠持续时间上存在有序交互，在日间功能障碍上存在无序交互。具体而言，在高工作压力下，G 等位基因携带者的睡眠持续时间比 AA 纯合子更差，而在低工作压力下，这两个亚组的睡眠持续时间相似，符合素质-应激模型。与 G 等位基因携带者相比，AA 纯合子在低工作压力下的日间功能障碍较少，而在高工作压力下的日间功能障碍较多，符合差异易感性模型。由于遗传联系已经被揭示，我们的研究可能有助于阐明睡眠质量的病因因素和实施干预措施以获得良好睡眠质量的目标。
