Brinza Ion, Abd-Alkhalek Ahmed M, El-Raey Mohamed A, Boiangiu Razvan Stefan, Eldahshan Omayma A, Hritcu Lucian
Department of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, Romania.
Faculty of Medicine, Al-Azhar University, Cairo 11651, Egypt.
Antioxidants (Basel). 2020 Jul 3;9(7):580. doi: 10.3390/antiox9070580.
Rhoifolin (Rho) exerts many biological activities such as anticancer, antidiabetic, hepatoprotective, antirheumatic, antibacterial, and antiviral properties. The neuroprotective action of this compound has not been studied. The goal of this study was to investigate the improvement impact of Rho on scopolamine (Sco)-induced zebrafish anxiety, amnesia, and brain oxidative stress and to elucidate the underlying mechanisms involved. Zebrafish were treated with Rho (1, 3, and 5 μg/L) for nine consecutive days and were subsequently subjected to Sco (100 μM) 30 min before behavioral tests (novel tank diving test, Y-maze, and novel object recognition tests). Rho was isolated from (Malvaceae) leaves and identified by different spectroscopic techniques. To further assess the possible mechanisms of Rho in enhancing the memory capacities in zebrafish, the in vivo antioxidant status and acetylcholinesterase (AChE) activity was also evaluated. Rho from leaves was identified. Rho could alleviate anxiety, memory deficits, and brain oxidative stress in Sco-treated zebrafish and could regulate the cholinergic function by inhibiting the AChE activity. Our results demonstrated that Rho could be a promising candidate compound against anxiety and amnesia by restoring the cholinergic activity and the amelioration of brain oxidative stress.
异鼠李素（Rho）具有多种生物活性，如抗癌、抗糖尿病、保肝、抗风湿、抗菌和抗病毒特性。该化合物的神经保护作用尚未得到研究。本研究的目的是探讨异鼠李素对东莨菪碱（Sco）诱导的斑马鱼焦虑、失忆及脑氧化应激的改善作用，并阐明其潜在机制。斑马鱼连续九天接受异鼠李素（1、3和5μg/L）处理，随后在行为测试（新水箱潜水试验、Y迷宫和新物体识别试验）前30分钟接受东莨菪碱（100μM）处理。异鼠李素从锦葵科植物的叶子中分离出来，并通过不同的光谱技术进行鉴定。为了进一步评估异鼠李素增强斑马鱼记忆能力的可能机制，还评估了体内抗氧化状态和乙酰胆碱酯酶（AChE）活性。从锦葵科植物叶子中鉴定出了异鼠李素。异鼠李素可以减轻东莨菪碱处理的斑马鱼的焦虑、记忆缺陷和脑氧化应激，并通过抑制乙酰胆碱酯酶活性来调节胆碱能功能。我们的结果表明，异鼠李素通过恢复胆碱能活性和改善脑氧化应激，可能是一种有前途的抗焦虑和抗失忆候选化合物。
