Capatina Luminita, Boiangiu Razvan Stefan, Dumitru Gabriela, Napoli Edoardo Marco, Ruberto Giuseppe, Hritcu Lucian, Todirascu-Ciornea Elena
Department of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, Romania.
Institute of Biomolecular Chemistry, National Research Council ICB-CNR, 95126 Catania, Italy.
Antioxidants (Basel). 2020 Jan 10;9(1):62. doi: 10.3390/antiox9010062.
L. is a traditional herb with various therapeutic applications such as antibacterial, antioxidant, anti-inflammatory, antidepressant, and anticholinesterase activities, and can be used for the prevention or treatment of dementia. In the present study, we tested whether L. could counteract scopolamine-induced anxiety, dementia, and brain oxidative stress in the zebrafish model and tried to find the underlying mechanism. L. essential oil (REO: 25, 150, and 300 µL/L) was administered by immersion to zebrafish () once daily for eight days while scopolamine (100 µM) treatment was delivered 30 min before behavioral tests. The antidepressant and cognitive-enhancing actions of the essential oil in the scopolamine zebrafish model was measured in the novel tank diving test (NTT) and Y-maze test. The chemical composition was identified by Gas chromatograph-Mass spectrometry (GC-MS) analysis. The brain oxidative status and acetylcholinesterase (AChE) activity was also determined. REO reversed scopolamine-induced anxiety, memory impairment, and brain oxidative stress. In addition, a reduced brain AChE activity following the administration of REO in scopolamine-treated fish was observed. In conclusion, REO exerted antidepressant-like effect and cognitive-enhancing action and was able to abolish AChE alteration and brain oxidative stress induced by scopolamine.
L. 是一种传统草药，具有多种治疗作用，如抗菌、抗氧化、抗炎、抗抑郁和抗胆碱酯酶活性，可用于预防或治疗痴呆症。在本研究中，我们测试了L. 是否能在斑马鱼模型中对抗东莨菪碱诱导的焦虑、痴呆和脑氧化应激，并试图找出其潜在机制。将L. 精油（REO：25、150和300 μL/L）通过浸泡法每天给斑马鱼施用一次，持续八天，而在行为测试前30分钟给予东莨菪碱（100 μM）治疗。在新水箱潜水试验（NTT）和Y迷宫试验中测量了精油在东莨菪碱斑马鱼模型中的抗抑郁和认知增强作用。通过气相色谱 - 质谱（GC-MS）分析确定了其化学成分。还测定了脑氧化状态和乙酰胆碱酯酶（AChE）活性。REO逆转了东莨菪碱诱导的焦虑、记忆障碍和脑氧化应激。此外，观察到在经东莨菪碱处理的鱼中施用REO后脑AChE活性降低。总之，REO发挥了类似抗抑郁的作用和认知增强作用，并且能够消除东莨菪碱诱导的AChE改变和脑氧化应激。
