Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
Coleman Technologies, Inc., Newtown Square, Pennsylvania, USA.
Nat Methods. 2014 Jun;11(6):625-8. doi: 10.1038/nmeth.2925. Epub 2014 Apr 13.
Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.
我们使用扫描后的激光导星和直接波前感应，演示了在高数值孔径（NA）和 14 毫秒更新率下对复杂像差的自适应校正。这种校正允许我们补偿生物样本中经常遇到的像差的快速空间变化，并在大体积（每边长>240 毫米）上恢复衍射极限成像。我们将其应用于斑马鱼大脑内精细神经元过程和亚细胞动力学的成像。
