Sausa Martina, Paladino Letizia, Scalia Federica, Zummo Francesco Paolo, Vergilio Giuseppe, Rappa Francesca, Cappello Francesco, Gratie Melania Ionelia, Proia Patrizia, Di Felice Valentina, Marino Gammazza Antonella, Macaluso Filippo, Barone Rosario
Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of Palermo, 90133 Palermo, Italy.
Department of Theoretical and Applied Sciences, eCampus University, 22060 Novedrate, Italy.
Nutrients. 2025 Apr 30;17(9):1550. doi: 10.3390/nu17091550.
: Recent studies have highlighted the role of the gut-muscle axis, suggesting that modulation of the gut microbiota may indirectly benefit skeletal muscle. This study aimed to evaluate the effects of () supplementation in a model of muscle atrophy induced by chronic ethanol (EtOH) intake, focusing on inflammatory and antioxidant mechanisms. : Sixty 12-month-old female Balb/c mice were divided randomly into three groups ( = 20/group): (1) Ethanol (EtOH) group, receiving ethanol daily for 8 and 12 weeks to induce systemic oxidative stress and inflammation; (2) Ethanol + Probiotic (EtOH + P) group, receiving both ethanol and supplementation for the same durations; and (3) Control (Ctrl) group, receiving only water. Muscle samples were analyzed for the fiber morphology, inflammatory markers, oxidative stress indicators, and satellite cell (SC) activity. All data were tested for normality using the Shapiro-Wilk test before applying a parametric analysis. A statistical analysis was performed using one-way ANOVA followed by a Bonferroni post-hoc test. The level of significance was set at < 0.05. : EtOH exposure caused significant atrophy in all muscle fiber types (type I, IIa, and IIb), with the most pronounced effects on oxidative fibers. supplementation significantly reversed atrophy in type I and IIa fibers, accompanied by a significant reduction in IL-6, TNF-α, and Hsp60 expression levels, indicating the protective effect of against oxidative stress and inflammation. Moreover, the probiotic treatment increased MyoD expression in SCs, suggesting enhanced regenerative activity, without histological evidence of fibrosis. : These findings suggest that supplementation could counteract EtOH-induced skeletal muscle damage by reducing inflammation and oxidative stress and promoting muscle repair, indicating its potential as an adjuvant, in the therapeutic strategy of models of muscle degeneration.
近期研究凸显了肠-肌轴的作用，表明调节肠道微生物群可能会间接有益于骨骼肌。本研究旨在评估（）补充剂在慢性乙醇（EtOH）摄入诱导的肌肉萎缩模型中的作用，重点关注炎症和抗氧化机制。：60只12月龄雌性Balb/c小鼠被随机分为三组（每组n = 20）：（1）乙醇（EtOH）组，连续8周和12周每日给予乙醇以诱导全身氧化应激和炎症；（2）乙醇+益生菌（EtOH + P）组，在相同时间段内同时给予乙醇和（）补充剂；（3）对照组（Ctrl），仅给予水。对肌肉样本进行纤维形态、炎症标志物、氧化应激指标和卫星细胞（SC）活性分析。在进行参数分析之前，使用Shapiro-Wilk检验对所有数据进行正态性检验。使用单因素方差分析，随后进行Bonferroni事后检验进行统计分析。显著性水平设定为P < 0.05。：EtOH暴露导致所有肌纤维类型（I型、IIa型和IIb型）出现明显萎缩，对氧化型纤维的影响最为显著。（）补充剂显著逆转了I型和IIa型纤维的萎缩，同时IL-6、TNF-α和Hsp60表达水平显著降低，表明（）对氧化应激和炎症具有保护作用。此外，益生菌治疗增加了SCs中MyoD的表达，表明再生活性增强，且无纤维化的组织学证据。：这些发现表明，（）补充剂可以通过减轻炎症和氧化应激以及促进肌肉修复来抵消EtOH诱导的骨骼肌损伤，表明其在肌肉退行性变模型治疗策略中作为佐剂的潜力。
