Kemp M, Husby S, Larsen M L, Svehag S E
Institute of Medical Microbiology, Odense University, Denmark.
Int Arch Allergy Appl Immunol. 1988;87(3):247-53. doi: 10.1159/000234680.
Enzyme immunoassays for the quantitation of IgA1 and IgA2 antibodies to dietary antigens were developed. Serum IgA1 antibodies to bovine serum albumin (BSA) were detectable in 2/30 healthy adults, in 3/26 healthy children, and at high levels in 8/11 children with coeliac disease, without relation to gluten exposure. IgA1 antibodies to ovalbumin (OA) and beta-lactoglobulin (BLG) at high titers were seen in one coeliac child but were otherwise low or absent. IgA2 antibodies to BSA were detectable in 28/48 healthy subjects and in 8/11 coeliac children. IgA2 antibodies to OA and BLG were measurable in a few samples from each group. IgA1 antibodies to the gluten component glycgli were found at low levels in 15/56 normal sera, and anti-glycgli antibodies of the IgA2 subclass in 14/48 sera from healthy persons, also at low levels. IgA1 anti-glycgli antibodies were measurable in 5/11 sera from CD patients on a gluten-free diet. Elevated levels of IgA1 anti-glycgli antibodies were detected in all sera from CD patients challenged with gluten, except in 1 patient with a markedly reduced serum IgA level. In contrast, the IgA2 anti-glycgli antibody levels were unaffected. Thus, increased levels of IgA antibodies to dietary protein antigens in childhood coeliac disease were observed only within the IgA1 isotype.
开发了用于定量检测针对饮食抗原的IgA1和IgA2抗体的酶免疫测定法。在2/30名健康成年人、3/26名健康儿童中可检测到针对牛血清白蛋白（BSA）的血清IgA1抗体，在8/11名乳糜泻儿童中该抗体水平较高，且与麸质暴露无关。在一名乳糜泻儿童中检测到高滴度的针对卵清蛋白（OA）和β-乳球蛋白（BLG）的IgA1抗体，其他儿童中该抗体水平较低或未检测到。在28/48名健康受试者和8/11名乳糜泻儿童中可检测到针对BSA的IgA2抗体。每组中少数样本可检测到针对OA和BLG的IgA2抗体。在15/56份正常血清中发现低水平的针对麸质成分麦醇溶蛋白的IgA1抗体，在14/48份健康人血清中发现IgA2亚类的抗麦醇溶蛋白抗体，水平也较低。在11名接受无麸质饮食的乳糜泻患者的5/11份血清中可检测到IgA1抗麦醇溶蛋白抗体。在所有接受麸质激发的乳糜泻患者血清中均检测到IgA1抗麦醇溶蛋白抗体水平升高，但有1名血清IgA水平明显降低的患者除外。相比之下，IgA2抗麦醇溶蛋白抗体水平未受影响。因此，仅在IgA1同种型中观察到儿童乳糜泻患者针对饮食蛋白抗原的IgA抗体水平升高。
