El-Sheekh Mostafa, Abu-Faddan Mahmoud, Abo-Shady Atef, Nassar Mohamed Zein Alabedin, Labib Wagdy
Botany Department, Faculty of Science, Tanta University, Tanta, Egypt.
Marine Environment Division, National Institute of Oceanography and Fisheries, Cairo, Egypt.
J Genet Eng Biotechnol. 2020 Jul 9;18(1):27. doi: 10.1186/s43141-020-00044-8.
An Egyptian indigenous unicellular green microalga was isolated from the coastal water of Suez Bay (N 29.92°, E 32.473°), Red Sea, Egypt. The molecular analysis based on 18S rRNA sequence showed that the gene sequence for this strain was highly similar (100% identity and 98% query cover) to different Chlorella strains isolated from different habitats.
The observed morphological characters together with the molecular phylogeny assigned the isolated microalga as Chlorella sp. MF1 with accession number KX228798. This isolated strain was cultivated for estimation of its growth and biochemical composition. The mean specific growth rate (μ) was 0.273 day. Both the biomass productivity and the cellular lipid content increased by increasing salinity of the growth medium, recording a maximum of 6.53 g l and 20.17%, respectively, at salinity 40.4. Fourteen fatty acids were identified. The total saturated fatty acid percentage was 54.73% with stearic (C18:0), arachidic (C20:0), and palmitic acids (C16:0) as major components, while the total unsaturated fatty acid percentage was 45.27% with linoleic acid (C18:2c) and oleic acid (C18:1) as majors.
This algal strain proved to be a potential newly introduced microalga as one of the most proper options available for microalgae-based biodiesel production. The proximate analysis showed the protein content at 39.85% and carbohydrate at 23.7%, indicating its accessibility to various purposes.
从埃及红海苏伊士湾沿海海域（北纬29.92°，东经32.473°）分离出一种埃及本土单细胞绿色微藻。基于18S rRNA序列的分子分析表明，该菌株的基因序列与从不同生境分离出的不同小球藻菌株高度相似（100% 一致性和98% 查询覆盖率）。
观察到的形态特征以及分子系统发育分析将分离出的微藻归为小球藻属MF1，登录号为KX228798。对该分离菌株进行培养以评估其生长和生化组成。平均比生长速率（μ）为0.273天⁻¹。随着生长培养基盐度的增加，生物量生产力和细胞脂质含量均增加，在盐度为40.4时分别达到最大值6.53 g/L和20.17%。鉴定出14种脂肪酸。总饱和脂肪酸百分比为54.73%，主要成分是硬脂酸（C18:0）、花生酸（C20:0）和棕榈酸（C16:0），而总不饱和脂肪酸百分比为45.27%，主要成分是亚油酸（C18:2c）和油酸（C18:1）。
该藻株被证明是一种潜在的新引入微藻，是基于微藻的生物柴油生产最适宜的可用选择之一。近似分析表明蛋白质含量为39.85%，碳水化合物含量为23.7%，表明其适用于多种用途。
