Department of Civil Engineering, Aalto University, Box 12100, FI-00076 Aalto, Finland.
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary.
Toxins (Basel). 2020 Jul 8;12(7):443. doi: 10.3390/toxins12070443.
The diversity of like isolates in buildings in Finland is poorly documented. This paper describes a set of methods for rapid diversity tracking of 42 indoor -like isolates. These isolates were categorized based on their fluorescence emission, ascomatal hair morphology, responses in three bioassays and resistance/sensitivity to the wetting agent Genapol X-080. Thirty-nine toxigenic isolates were identified [ ( = 35), ( = 2) and ( = 2)]. These isolates were identified down to the species level by gene sequencing. The major toxic substances in the ethanol extracts of the and strains were chaetoglobosin, chaetoviridin A and C, chaetomugilin D and chaetomin, identified based on HPLC-UV and mass spectrometry data (MS and MS/MS). Ethanol extracts from pure cultures exhibited a toxicological profile in the boar sperm motility inhibition assay (BSMI), sperm membrane integrity damage assay (SMID) and inhibition of cell proliferation (ICP) assay, similar to that exhibited by pure chaetoglobosin A. Overall, differences in fluorescence, morphology, toxicity profile, mycotoxin production and sensitivity to chemicals were consistent with those in sequencing results for species identification. The results indicate the presence of and in Finnish buildings, representing a new finding.
芬兰建筑物中类似菌株的多样性记录很少。本文描述了一套快速追踪 42 种室内类似菌株多样性的方法。这些菌株根据其荧光发射、子囊壳毛形态、三种生物测定中的反应以及对润湿剂 Genapol X-080 的抗性/敏感性进行了分类。确定了 39 个产毒菌株[ ( = 35)、( = 2)和 ( = 2)]。这些菌株通过基因测序鉴定到种的水平。从 和 菌株的乙醇提取物中，根据 HPLC-UV 和质谱数据 (MS 和 MS/MS)，鉴定出了 chaetoglobosin、chaetoviridin A 和 C、chaetomugilin D 和 chaetomin 等主要有毒物质。纯 培养物的乙醇提取物在公猪精子运动抑制试验 (BSMI)、精子膜完整性损伤试验 (SMID)和细胞增殖抑制试验 (ICP)中表现出与纯 chaetoglobosin A 相似的毒理学特征。总的来说，荧光、形态、毒性特征、产毒和对化学物质的敏感性差异与种鉴定的测序结果一致。结果表明，芬兰建筑物中存在 和 ，这是一个新的发现。
