Zhou Jerry, Shrestha Prapti, Qiu Zhiguang, Harman David G, Teoh Wun-Chung, Al-Sohaily Sam, Liem Han, Turner Ian, Ho Vincent
School of Medicine, Western Sydney University, Campbelltown, NSW 2560, Australia.
Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW 2750, Australia.
J Clin Med. 2020 Jul 8;9(7):2162. doi: 10.3390/jcm9072162.
Non-erosive reflux disease (NERD) and esophageal adenocarcinoma (EAC) are often regarded as bookends in the gastroesophageal reflux disease spectrum. However, there is limited clinical evidence to support this disease paradigm while the underlying mechanisms of disease progression remain unclear. In this study, we used 16S rRNA sequencing and mass-spectrometer-based proteomics to characterize the esophageal microbiota and host mucosa proteome, respectively. A total of 70 participants from four patient groups (NERD, reflux esophagitis, Barrett's esophagus, and EAC) and a control group were analyzed. Our results showed a unique NERD microbiota composition, distinct to control and other groups. We speculate that an increase in sulfate-reducing Proteobacteria and Bacteroidetes along with hydrogen producer are associated with a mechanistic role in visceral hypersensitivity. We also observed a distinct EAC microbiota consisting of a high abundance of lactic acid-producing bacteria (, , , and ), which may contribute towards carcinogenesis through dysregulated lactate metabolism. This study suggests the close relationship between esophageal mucosal microbiota and the appearance of pathologies of this organ.
非糜烂性反流病（NERD）和食管腺癌（EAC）通常被视为胃食管反流病谱中的两端。然而，支持这种疾病模式的临床证据有限，而疾病进展的潜在机制仍不清楚。在本研究中，我们分别使用16S rRNA测序和基于质谱的蛋白质组学来表征食管微生物群和宿主黏膜蛋白质组。对来自四个患者组（NERD、反流性食管炎、巴雷特食管和EAC）和一个对照组的70名参与者进行了分析。我们的结果显示了独特的NERD微生物群组成，与对照组和其他组不同。我们推测，硫酸盐还原变形菌和拟杆菌以及氢气产生菌的增加在内脏超敏反应中具有机制性作用。我们还观察到一种独特的EAC微生物群，其由高丰度的产乳酸菌（、、和）组成，这可能通过乳酸代谢失调促进致癌作用。这项研究表明食管黏膜微生物群与该器官病变的出现密切相关。
