生物炭影响猪粪中抗生素的降解和抗生素抗性基因的丰度。
Biochar affects the dissipation of antibiotics and abundance of antibiotic resistance genes in pig manure.
机构信息
Soil Science, University of Trier, Trier, Germany; Department of Chemistry, Multimedia University of Kenya, Nairobi, Kenya.
Korea Biochar Research Center, APRU Sustainable Waste Management & Division of Environmental Science and Ecological Engineering, Korea University, Seoul, Republic of Korea.
出版信息
Bioresour Technol. 2020 Nov;315:123782. doi: 10.1016/j.biortech.2020.123782. Epub 2020 Jul 6.
Manure from medicated livestock contains pharmaceutical antibiotics and antibiotic resistance genes (ARGs). Bioavailable antibiotics trigger further ARGs amplification during manure storage. It was tested whether biochar lowers the bioavailability of the antibiotics sulfamethazine (SMZ), ciprofloxacin (CIP), oxytetracycline (OTC) and florfenicol (FF) in manure and the amplification of sul1 and tet(W) ARGs. To that end, liquid pig manure was treated with 5% (w/w) pinecone biochar (BCP). Antibiotics dissipated during 30-d incubation in the order SMZ < OTC < CIP < FF. Added BCP further immobilized SMZ, OTC and CIP, while the effect was not significant for FF. Both sul1 and tet(W) ARGs copy numbers significantly increased by factors of 5.8 and 2.5, respectively, in OTC and SMZ spiked manure. The abundance of sul1 was significantly decreased in BCP amended manure, while the impact on tet(W) was less. Consequently, biochar is suitable for the management of antibiotics contaminated manure during storage.
来自用药牲畜的粪便中含有药物抗生素和抗生素抗性基因（ARGs）。在粪便储存过程中，生物可利用的抗生素会引发进一步的 ARGs 扩增。本研究测试了生物炭是否能降低粪便中磺胺甲噁唑（SMZ）、环丙沙星（CIP）、土霉素（OTC）和氟苯尼考（FF）等抗生素以及 sul1 和 tet(W) ARGs 的生物可利用性和扩增。为此，将 5%（w/w）的松果生物炭（BCP）添加到猪粪中。在 30 天的孵育过程中，抗生素的消解顺序为 SMZ < OTC < CIP < FF。添加 BCP 进一步固定了 SMZ、OTC 和 CIP，但对 FF 的影响不显著。在添加 OTC 和 SMZ 的粪便中，sul1 和 tet(W) ARGs 的拷贝数分别显著增加了 5.8 倍和 2.5 倍。在添加 BCP 的粪便中，sul1 的丰度显著降低，而对 tet(W) 的影响较小。因此，生物炭适合在储存过程中管理抗生素污染的粪便。
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